Isolation rearing and hyperlocomotion are associated with reduced immediate early gene expression levels in the medial prefrontal cortex
Isolation rearing and hyperlocomotion are associated with reduced immediate early gene expression levels in the medial prefrontal cortex
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DOI：
10.1016/j.neuroscience.2006.11.063
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发表时间：
2007-03-02
期刊：
影响因子：
3.3
通讯作者：
Konradi, C.
中科院分区：
文献类型：
--
作者：
Levine, J. B.;Youngs, R. M.;Konradi, C.
环境剥夺在很大程度上促成了一系列精神疾病的发展。隔离饲养的啮齿动物，在人类的环境剥夺的模型，始终产生hypermotorism，这提供了一个可测量的参数，以研究早期不良心理社会应激的潜在机制。在出生后（PN）第20天将雄性Sprague-Dawley大鼠幼仔与母鼠分开，并以三只一组或单独饲养。PN 38时，在开放视野中评估运动。PN 46时，处死大鼠，检测内侧前额叶皮质（mPFC）中的基因表达模式。隔离饲养的大鼠表现出增加的自发活动和减少休息时间在开放领域。mPFC中的特定基因表达模式与隔离饲养和开阔场地中的过度运动行为相关。参与这些表达模式的基因包括立即早期基因（IEGs）和调节细胞分化和凋亡的基因。对这些基因的研究可以为了解异常的早期心理社会事件如何影响大脑功能和行为提供重要的见解。(c)2006年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Environmental deprivation contributes in important ways to the development of a wide range of psychiatric disorders. Isolation rearing of rodents, a model for environmental deprivation in humans, consistently produces hyperlocomotion, which provides a measurable parameter to study the underlying mechanisms of early adverse psychosocial stressors. Male Sprague-Dawley rat pups were separated from dams at postnatal (PN) day 20 and reared either in groups of three or in isolation. On PN 38, locomotion was assessed in the open field. On PN 46, rats were killed and gene expression patterns examined in the medial prefrontal cortex (mPFC). Isolation-reared rats displayed increased locomotor activity and decreased resting time in the open field. Specific gene expression patterns in the mPFC were associated with both isolation rearing and hyperlocomotive behavior in the open field. Genes involved in these expression patterns included immediate early genes (IEGs) and genes that regulate cell differentiation and apoptosis. The study of these genes could provide important insights into how abnormal early psychosocial events affect brain function and behavior. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.