GOLGA4, A Golgi matrix protein, is dispensable for spermatogenesis and male fertility in mice
GOLGA4, A Golgi matrix protein, is dispensable for spermatogenesis and male fertility in mice
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DOI：
10.1016/j.bbrc.2020.05.170
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发表时间：
2020-08-27
影响因子：
3.1
通讯作者：
Yuan, Shuiqiao
中科院分区：
文献类型：
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作者：
Guo, Shuangshuang;Lv, Chunyu;Yuan, Shuiqiao
在顶体生物发生过程中，许多颗粒形成的trans-Golgi堆栈和积累在凹区的核表面，是必要的顶体形成。一些高尔基体相关蛋白参与了这一过程。然而，高尔基体相关蛋白，特别是高尔基体基质蛋白，在顶体生物发生中的具体功能仍然是一个谜。在这项研究中，我们确定GOLGA 4，作为高尔基体基质蛋白，在小鼠睾丸中高度表达。为了探索GOLGA 4在精子发生中的功能，我们使用CRISPR/Cas9技术建立了Golga 4全基因敲除小鼠系，并证明Golga 4敲除雄性具有正常的睾丸和精子形态。此外，睾丸组织学显示WT和KO小鼠之间无显著差异。总之，我们的数据表明，GOLGA 4是小鼠精子发生和雄性生育能力。(C)2020由Elsevier Inc.出版。
During acrosome biogenesis, numerous granules formed from trans-Golgi stacks and accumulated in the concave region of the nuclear surface that is essential for acrosome formation. Several Golgi-associated proteins were involved in this process. However, the specific function of Golgi-associated proteins, especially Golgi matrix protein, during acrosome biogenesis remains elusive. In this study, we identified GOLGA4, as a Golgi matrix protein, highly expressed in mouse testes. To explore the function of GOLGA4 in spermatogenesis, we generated a Golga4 global knockout mouse line using CRISPR/Cas9 technology and demonstrated that Golga4 knockout males are fertile with normal morphology of testis and sperm. Furthermore, testicular histology showed no significant difference between WT and KO mice. Together, our data demonstrate that GOLGA4 is dispensable for mouse spermatogenesis and male fertility. (C) 2020 Published by Elsevier Inc.