Physiological and transcriptional responses to low-temperature stress in rice genotypes at the reproductive stage
Physiological and transcriptional responses to low-temperature stress in rice genotypes at the reproductive stage
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DOI：
10.1080/15592324.2019.1581557
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发表时间：
2019-04-03
影响因子：
2.9
通讯作者：
Pereira, Andy
中科院分区：
文献类型：
--
作者：
Moraes de Freitas, Gabriela Peres;Basu, Supratim;Pereira, Andy
低温胁迫对水稻生长和产量产生不利影响。从以前的筛选不同的水稻基因型在营养阶段的耐冷性参数，我们选择了耐日本晴和M202基因型和敏感的柏树和Secano做巴西基因型在生殖阶段的生理和产量参数进行进一步分析。冷胁迫严重影响每穗粒数、穗长和百粒重等产量指标。对这些基因型的旗叶和花序组织中参与对低温的生理反应的21个基因的基因表达进行分析，结果表明，在耐低温的日本晴和M202的旗叶组织中，脂质转移蛋白基因LTP7和LTP10的表达增加，沿着胁迫应答转录因子（TF）和冷诱导基因的相对表达的显著增加。在剑叶组织中，OsNAC9、OsNAC10和OsNAP基因与光合作用、气孔导度、蒸腾作用和PSII量子效率高度相关。根据以上结果，我们得出结论，日本晴和M202是耐冷基因型，并且LTP7、LTP10、OsNAC9、OsNAC10和OsNAP基因可以用作生殖期耐冷性筛选的标记。此外，基于这些结果，我们提出了一个模型的低温耐受机制如何感知压力，以及如何在低于理想温度的信号级联作用，以促进耐受性。
Rice growth and productivity is adversely affected by low-temperature stress. From a previous screen of diverse rice genotypes for cold tolerance parameters at the vegetative stage, we selected the tolerant Nipponbare and M202 genotypes and sensitive Cypress and Secano do Brazil genotypes for further analysis at the reproductive stage for physiological and yield parameters. Cold stress severely affected grain yield as estimated by the number of grain per panicle, panicle length, and 100 seed weight. Analysis of gene expression of 21 genes involved in physiological responses to low temperature tested, in the flag leaf and inflorescence tissue of these genotypes, showed an increased expression of the Lipid Transfer Protein genes LTP7 and LTP10 in flag leaf tissue of the tolerant Nipponbare and M202, along with a significant increase in the relative expression of stress-responsive transcription factors (TFs) and cold-inducible genes. In flag leaf tissue OsNAC9, OsNAC10 and OsNAP genes showed high correlation with photosynthesis, stomatal conductance, transpiration and Quantum Efficiency of PSII. In consequence of the foregoing results, we conclude that Nipponbare and M202 are cold tolerant genotypes and that LTP7, LTP10, OsNAC9, OsNAC10 and OsNAP genes can be used as markers in screening for cold tolerance at the reproductive stage. Furthermore based on the results we propose a model of low-temperature tolerance mechanism of how stress is perceived, and how the signal cascade acts to promote tolerance at below-ideal temperatures.