Cell configuration-related control of vimentin biosynthesis and phosphorylation in cultured mammalian cells.
Cell configuration-related control of vimentin biosynthesis and phosphorylation in cultured mammalian cells.
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DOI：
10.1083/jcb.97.3.858
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发表时间：
1983-09
影响因子：
7.8
通讯作者：
Ben-Ze'ev, A
中科院分区：
文献类型：
--
作者：
Ben-Ze'ev, A
通过在扁平和球形之间改变细胞形状，研究了细胞形态对细胞骨架蛋白(Vimentin)表达的控制。在聚甲基丙烯酸羟乙酯包被的平板上单层或球形培养细胞发现，在悬浮培养过程中，波形蛋白的合成被优先下调。S调节球形细胞Vimentin表达下降的机制似乎是在翻译水平，因为从单层细胞和悬浮培养细胞中提取的mRNAs在引导兔网织红细胞无细胞系统中Vimentin合成方面具有同等的活性。在长时间的悬浮培养后，允许细胞重新贴壁和铺展，波形蛋白合成迅速恢复到对照单层速率。在悬浮培养过程中，波形蛋白的磷酸化程度也显著降低。然而，与重新附着时波形蛋白生物合成的快速恢复不同(不到6h)，波形蛋白磷酸化的恢复速度要慢得多(大于20h)，并与单层生长速度平行。虽然悬浮培养中波形蛋白生物合成的控制是一个与细胞形态有关的过程，但悬浮培养中波形蛋白磷酸化速率的降低似乎是生长速度较慢的结果，可能反映了已报道的波形蛋白磷酸化速率与细胞有丝分裂积累之间的相关性。
The cell configuration-related control of a cytoskeletal protein (vimentin) expression was examined by varying cell shape between flat and spherical. Cultivation of cells in monolayer or in a spherical configuration on poly-2-hydroxyethylmethacrylate-coated plates revealed a preferential down regulation of vimentin synthesis during suspension culture. The mechanism(s) regulating the decrease in the expression of vimentin in spherical cells appears to be at the level of translation, because mRNAs extracted from monolayer and suspension-cultured cells were equally active in directing vimentin synthesis in the rabbit reticulocyte cell-free system. When after prolonged suspension culture, the cells were allowed to reattach and spread, vimentin synthesis recovered rapidly to the control monolayer rate. The phosphorylation of vimentin was also reduced dramatically during suspension culture. However, unlike the rapid recovery of vimentin biosynthesis upon reattachment (less than 6 h), the recovery in the rate of vimentin phosphorylation was much slower (greater than 20 h) and paralleled the recovery to the monolayer growth rate. Although the control of vimentin biosynthesis in suspension culture is a cell configuration-related process, the decrease in the rate of vimentin phosphorylation in suspension culture appears to be the result of the slower growth rate and may reflect the reported correlation between the rate of vimentin phosphorylation and the accumulation of cells in mitosis.