Motor Properties of PilT-Independent Type 4 Pilus Retraction in Gonococci
Motor Properties of PilT-Independent Type 4 Pilus Retraction in Gonococci
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淋球菌中不依赖 PilT 的 4 型菌毛回缩的运动特性
DOI：
10.1128/jb.00778-18
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发表时间：
2019
影响因子：
3.2
通讯作者：
Cronenberg
中科院分区：
文献类型：
--
作者：
Zöllner;Cronenberg
细菌4型皮利（T4 P）属于最强的分子机器。淋球菌T4 P收缩ATP酶PilT在T4 P收缩期间支持超过100 pN的力。在这里，我们解决的问题是否淋球菌T4P缩回PilT的情况下。我们发现pilT缺失菌株确实收缩了它们的T4P，但最大力降低到5 pN。类似地，与由PilT驱动的T4P回缩的速度相比，T4P回缩的速度低几个数量级。删除pilTparaloguepilT2进一步降低了T4P收缩的速度，但在所有三个epilTparaloguepilT2缺失的情况下，T4P收缩是可检测的。此外，我们表明，质子动力（PMF）的耗尽减慢，但不抑制pilT独立的T4P收缩。我们的结论是，收缩ATP酶是不是必不可少的淋球菌T4P收缩。重要提示细菌4型皮利（T4 P）被称为细菌的“瑞士军刀”，因为它们执行许多功能，包括宿主细胞相互作用、抽搐运动、集落形成、DNA摄取、蛋白质分泌，和表面传感。菌毛纤维不断地伸长或收缩，这些动力学在功能上是重要的。奇怪的是，只有一部分T4P系统使用T4P收缩ATP酶来为T4P收缩提供动力。在这里，我们表明，最强的T4P机器之一，淋球菌T4P，缩回没有回缩ATP酶。生物物理表征显示，与ATP酶牵开相比，力和速度大大降低。我们提出，细菌编码收缩ATP酶时，T4 P必须产生高力支持功能，如抽搐运动，触发宿主细胞反应，或流化殖民地。
Bacterial type 4 pili (T4P) belong to the strongest molecular machines. The gonococcal T4P retraction ATPase PilT supports forces exceeding 100 pN during T4P retraction. Here, we address the question of whether gonococcal T4P retract in the absence of PilT. We show thatpilTdeletion strains indeed retract their T4P, but the maximum force is reduced to 5 pN. Similarly, the speed of T4P retraction is lower by orders of magnitude compared to that of T4P retraction driven by PilT. Deleting thepilTparaloguepilT2further reduces the speed of T4P retraction, yet T4P retraction is detectable in the absence of all threepilTparalogues. Furthermore, we show that depletion of proton motive force (PMF) slows but does not inhibitpilT-independent T4P retraction. We conclude that the retraction ATPase is not essential for gonococcal T4P retraction. However, the force generated in the absence of PilT is too low to support important functions of T4P, including twitching motility, fluidization of colonies, and induction of host cell response.IMPORTANCEBacterial type 4 pili (T4P) have been termed the “Swiss Army knives” of bacteria because they perform numerous functions, including host cell interaction, twitching motility, colony formation, DNA uptake, protein secretion, and surface sensing. The pilus fiber continuously elongates or retracts, and these dynamics are functionally important. Curiously, only a subset of T4P systems employ T4P retraction ATPases to power T4P retraction. Here, we show that one of the strongest T4P machines, the gonococcal T4P, retracts without a retraction ATPase. Biophysical characterization reveals strongly reduced force and speed compared to retraction with ATPase. We propose that bacteria encode retraction ATPases when T4P have to generate high-force-supporting functions like twitching motility, triggering host cell response, or fluidizing colonies.
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影响因子：
16.6
作者：
McCallum M;Tammam S;Khan A;Burrows LL;Howell PL
通讯作者：
Howell PL
影响因子：
6.7
作者：
Gangel H;Hepp C;Müller S;Oldewurtel ER;Aas FE;Koomey M;Maier B
通讯作者：
Maier B
分子马达控制细菌菌落的液体排序和融合动力学
影响因子：
8.6
作者：
Welker;Cronenberg;Zöllner;Siewering;Bender;Hennes;Oldewurtel
通讯作者：
Oldewurtel
影响因子：
5.7
作者：
Mancl, Jordan M.;Black, Wesley P.;Schubot, Florian D.
通讯作者：
Schubot, Florian D.
影响因子：
4.6
作者：
Zöllner R;Oldewurtel ER;Kouzel N;Maier B
通讯作者：
Maier B