Chronic inhalation of nitric oxide inhibits neointimal formation after balloon-induced arterial injury
Chronic inhalation of nitric oxide inhibits neointimal formation after balloon-induced arterial injury
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DOI：
10.1161/01.res.78.2.337
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发表时间：
1996-02-01
影响因子：
20.1
通讯作者：
Bloch, KD
中科院分区：
文献类型：
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作者：
Lee, JS;Adrie, C;Bloch, KD
在动物模型中全身和局部血管内注射NO抑制血管损伤后新生内膜的形成。NO似乎通过阻止生长因子的释放而直接或间接地抑制血管的增殖。吸入低浓度的NO可扩张肺血管平滑肌，但不会引起全身血管扩张。近年来，在体内发现吸入NO对血小板功能有抑制作用。我们研究了吸入NO对成年大鼠颈动脉球囊损伤后新生内膜形成的影响。从颈动脉损伤前60分钟开始，大鼠连续14天呼吸含0或80ppm NO的空气。处死大鼠，固定颈动脉，石蜡包埋，通过分析颈动脉横断面内膜/中膜面积的比率(I/M比)来测量新生内膜的形成。两组动物均有明显的内膜增生，但呼吸80ppm NO 2周的I/M比仅呼吸空气的动物低43%(分别为0.78+/-0.12和1.37+/-0.11[Mean+/-SE]，P
Systemic and local intravascular NO administration inhibits neointimal formation after vascular injury in animal models. NO appears to attenuate smooth muscle proliferation both directly and indirectly by preventing the release of growth factors. Inhalation of low concentrations of NO dilates pulmonary vascular smooth muscle but does not cause systemic vasodilatation. Recently, NO inhalation was found to inhibit platelet function in vivo. We studied the effects of NO inhalation on neointimal formation after balloon-induced injury of the adult rat carotid artery. Beginning 60 minutes before carotid injury, rats breathed either air with 0 or 80 ppm NO for 14 days. Rats were killed, carotid arteries were fixed and paraffin-embedded, and neointimal formation was measured by analyzing the ratio of intimal to medial areas (I/M ratio) in carotid artery cross sections. Intimal hyperplasia was evident in both groups of animals, but I/M ratios were 43% less in animals breathing 80 ppm NO for 2 weeks than in animals breathing air alone (0.78+/-0.12 and 1.37+/-0.11 [mean+/-SE], respectively; P