The red sport of 'Zaosu' pear and its red-striped pigmentation pattern are associated with demethylation of the PyMYB10 promoter
The red sport of 'Zaosu' pear and its red-striped pigmentation pattern are associated with demethylation of the PyMYB10 promoter
复制标题
‘早酥’梨的红色运动及其红色条纹色素沉着模式与 PyMYB10 启动子的去甲基化有关
DOI：
10.1016/j.phytochem.2014.08.001
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发表时间：
2014-11-01
期刊：
影响因子：
3.8
通讯作者：
Zhang, Dong
中科院分区：
文献类型：
--
作者：
Qian, Minjie;Sun, Yongwang;Zhang, Dong
早酥梨是由砂梨和鸭梨杂交而成的梨新品种，在我国是一个很受欢迎的栽培品种。‘早苏红’是‘早苏’的芽变，具有红色的枝条、嫩叶和果实。早苏红嫁接后，部分枝条发生回复突变，导致叶片和茎部颜色丧失。此外，'早苏红'的成熟果实表现出两种表型：全红和条纹。本研究旨在探讨早苏红的红色突变机理和果实条纹状色素形成模式。花色素苷的积累与PpUFGT 2和PyMYB 10基因的转录水平高度相关。克隆了这两个关键基因的开放阅读框（ORF）和启动子区，并与“早苏”和其芽体进行了比较，结果表明：R2 R3 MYB（PyMYB 10）能够激活花青素合成途径相关酶基因的表达。酵母单杂交分析表明PyMYB 10与PpUFGT 2启动子的-658 ~-172 bp片段结合，可能是通过位于-466 bp的MYB结合位点（MBS）。PyMYB 10启动子在富含花青素的组织中甲基化水平较低，表明早酥梨的红芽运动和早酥梨的条纹状着色模式与PyMYB 10启动子的去甲基化有关。(C)2014爱思唯尔有限公司版权所有。
'Zaosu' pear, a hybrid of Pyrus pyrifolia and Pyrus communis, is a popular cultivar developed in China. 'Zaosu Red' is a bud sport of 'Zaosu' with red shoots, young leaves, and fruit. After grafting of 'Zaosu Red', reverse mutations in some branches lead to a loss of colour in leaves and stems. Also, the mature fruit of 'Zaosu Red' exhibits two phenotypes; fully red and striped. The aim of this study was to establish the mechanism of the red colour mutation in 'Zaosu' and the striped pigmentation pattern in fruit of 'Zaosu Red'. The accumulation of anthocyanins and transcript levels of the genes PpUFGT2 and PyMYB10 were highly correlated. The open reading frames (ORF) and promoter regions of these two key genes were cloned and compared between 'Zaosu' and its bud sports, but no sequence differences were found. The R2R3 MYB, PyMYB10, can activate expression of genes encoding enzymes of the anthocyanin biosynthetic pathway. A yeast one-hybrid assay showed that PyMYB10 was associated with the -658 to -172 bp fragment of the PpUFGT2 promoter, probably via a MYB binding site (MBS) located at -466 bp. The PyMYB10 promoter had lower methylation levels in anthocyanin-rich tissues, indicating that the red bud sport of 'Zaosu' pear and the striped pigmentation pattern of 'Zaosu Red' pear are associated with demethylation of the PyMYB10 promoter. (C) 2014 Elsevier Ltd. All rights reserved.