Effect of 5' and 3' terminal sequences, overall length, and coding capacity on the accumulation of defective RNAs associated with broad bean mottle bromovirus in planta.
Effect of 5' and 3' terminal sequences, overall length, and coding capacity on the accumulation of defective RNAs associated with broad bean mottle bromovirus in planta.
复制标题
5 和 3 末端序列、总长度和编码能力对植物中与蚕豆斑驳溴病毒相关的缺陷 RNA 积累的影响。
DOI：
10.1006/viro.1996.8377
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Bujarski,JJ
中科院分区：
文献类型：
--
作者：
Pogany,J;Romero,J;Bujarski,JJ
蚕豆斑驳溴病毒（BBMV）显示积累RNA 2衍生的缺陷型干扰（DI）RNA [Romeroet al.，Virology 194，576-584（1993）; Poganyet al.，病毒学212，574-586（1995）]。在本研究中，我们利用三组BBMV RNA 2衍生的人工DI RNA构建体来确定影响缺陷RNA在植物中积累的因素，其中一组缺失构建体用于定位DI RNA的5′ 883 nt和3′ 387 nt内有效积累所需的序列。第二组具有逐渐增加的3′嵌套缺失的大小，以确定有效DI RNA积累所需的最小长度。发现仍在植物中积累的最小DI RNA为1712 nt长。第三组由移码突变体组成，其显示至少64.4%的BBMV DI RNA序列必须具有2a开放阅读框的5′部分以确保有效积累。这些因素在DI RNA的选择的重要性进行了讨论。
Broad bean mottle bromovirus (BBMV) was shown to accumulate RNA2-derived defective interfering (DI) RNAs [Romeroet al., Virology194, 576–584 (1993); Poganyet al., Virology212, 574–586 (1995)]. In this work, we utilize three sets of BBMV RNA2-derived artificial DI RNA constructs to determine factors that affect the accumulation of defective RNAsin planta.One set of deletion constructs was used to localize sequences required for efficient accumulation within the 5′ 883 nt and the 3′ 387 nt of the DI RNAs. The second set had a gradually increasing size of 3′ nested deletions to determine the minimal length required for efficient DI RNA accumulation. The smallest DI RNA still accumulating in plants was found to be 1712 nt long. The third set consisted of frameshift mutants which showed that at least 64.4% of BBMV DI RNA sequences must have the 5′ portion of the 2a open reading frame to ensure efficient accumulation. The importance of these factors in the selection of DI RNAs is discussed.