Interspecies communication between plant and mouse gut host cells through edible plant derived exosome-like nanoparticles.
Interspecies communication between plant and mouse gut host cells through edible plant derived exosome-like nanoparticles.
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DOI：
10.1002/mnfr.201300729
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发表时间：
2014-07
影响因子：
5.2
通讯作者：
Zhang HG
中科院分区：
文献类型：
--
作者：
Mu J;Zhuang X;Wang Q;Jiang H;Deng ZB;Wang B;Zhang L;Kakar S;Jun Y;Miller D;Zhang HG
外泌体是一种参与细胞间通讯的小泡囊，近年来已被广泛研究;然而，可食用植物来源的外泌体在种间通讯中的作用尚未被研究。在这里，我们研究了可食用植物来源的外泌体样纳米颗粒（EPDEN）对哺乳动物细胞的生物学效应。在这项研究中，从四种可食用植物中分离出外泌体样纳米颗粒并进行了表征。我们发现，这些EPDENs含有蛋白质，脂质和microRNA。EPDEN被肠道巨噬细胞和干细胞吸收。从EPDEN转染的巨噬细胞产生的结果表明，生姜EPDEN优先诱导抗氧化基因血红素加氧酶-1（HO-1）和抗炎细胞因子IL-10的表达;而葡萄柚、生姜和胡萝卜EPDEN促进核因子（红细胞衍生2）样2（Nrf 2）的活化。此外，分析典型Wnt报告小鼠的肠，即，B6.Cg-Tg（BAT-lacZ）3 Picc/J小鼠的结果显示，β-半乳糖苷酶+（β-Gal）肠隐窝的数量增加，表明小鼠的EPDEN处理导致隐窝中Wnt介导的Tcf 4转录机制的激活。这些数据表明，EPDEN介导的种间通信的作用，通过诱导抗炎细胞因子，抗氧化和激活Wnt信号的基因的表达，这是至关重要的维持肠道内稳态。
Exosomes, small vesicles participating in intercellular communication have been extensively studied recently; however, the role of edible plant derived exosomes in interspecies communication has not been investigated. Here, we investigate the biological effects of edible plant derived exosome-like nanoparticles (EPDEN) on mammalian cells. In this study, exosome-like nanoparticles from four edible plants were isolated and characterized. We show that these EPDENs contain proteins, lipids and microRNA. EPDENs are taken up by intestinal macrophages and stem cells. The results generated from EPDEN transfected macrophages indicate that ginger EPDENs preferentially induce the expression of the anti-oxidation gene, heme oxygenase-1 (HO-1) and the anti-inflammatory cytokine, IL-10; whereas grapefruit, ginger, and carrot EPDENs promote activation of nuclear factor (erythroid-derived 2)-like 2 (Nrf2). Furthermore, analysis of the intestines of canonical Wnt reporter mice, i.e., B6.Cg-Tg(BAT-lacZ)3Picc/J mice, revealed that the numbers of β-galactosidase+ (β-Gal) intestinal crypts are increased, suggesting that EPDEN treatment of mice leads to Wnt mediated activation of the Tcf4 transcription machinery in the crypts. The data suggest a role for EPDEN mediated interspecies communication by inducing expression of genes for anti-inflammation cytokines, anti-oxidation and activation of Wnt signaling, which are crucial for maintaining intestinal homeostasis.