Expression of alpha 6 and beta 4 integrins in serous ovarian carcinoma correlates with expression of the basement membrane protein laminin.
Expression of alpha 6 and beta 4 integrins in serous ovarian carcinoma correlates with expression of the basement membrane protein laminin.
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发表时间：
1996
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通讯作者：
A. Skubitz;R. Bast;E. Wayner;Paul C. Letourneau;M. S. Wilke
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作者：
A. Skubitz;R. Bast;E. Wayner;Paul C. Letourneau;M. S. Wilke
正常卵巢的表面由单层上皮细胞覆盖，上皮细胞位于基底膜上。糖蛋白层粘连蛋白是存在于基底膜中的主要非胶原蛋白。整联蛋白α 1 β 1、α 2 β 1、α 3 β 1、α 6 β 1和α 6 β 4作为层粘连蛋白的细胞表面受体。在浆液性卵巢癌的进展过程中，肿瘤细胞经常从卵巢表面脱落，从而失去与基底膜的接触。本研究旨在确定整合素表达的改变是否与原发性卵巢肿瘤和腹水中脱落的卵巢癌细胞的恶性表型相关。通过免疫组织化学染色，正常卵巢上皮细胞的整个表面对β 1、α 2和α 3整合素染色阳性，而只有上皮细胞的基底表面，在那里它们与层粘连蛋白接触，对α 6和β 4染色阳性。浆液性卵巢癌患者实体瘤上皮细胞的整个表面β 1、α 2和α 3整合素染色呈阳性。在大多数情况下，肿瘤巢周围没有完整的基底膜，α 6和β 4染色不规则。当存在时，基底膜层粘连蛋白染色阳性，上皮细胞的基底表面α 6和β 4染色阳性。卵巢癌腹水细胞表现出独特的表型，α 6和β 4整合素亚基的表达显著降低。由于α 6和β 4整联蛋白亚基存在于许多上皮细胞的基底表面，并作为层粘连蛋白的受体，因此卵巢癌上皮细胞可能由于其α 6和β 4整联蛋白亚基的缺陷而从卵巢的基底膜释放。
The surface of a normal ovary is covered by a monolayer of epithelial cells that rest on a basement membrane. The glycoprotein laminin is the major noncollagenous protein present in the basement membrane. The integrins alpha 1 beta 1, alpha 2 beta 1, alpha 3 beta 1, alpha 6 beta 1, and alpha 6 beta 4 serve as cell surface receptors for laminin. During the progression of serous ovarian carcinoma, tumor cells are frequently exfoliated from the surface of the ovary, thereby losing contact with the basement membrane. This study was designed to determine whether alterations in integrin expression may be associated with the malignant phenotype of the primary ovarian tumor and exfoliated ovarian carcinoma cells in the ascites fluid. By immunohistochemical staining, the entire surface of epithelial cells of normal ovaries stained positively for beta 1, alpha 2, and alpha 3 integrins, whereas only the basal surface of the epithelial cells, where they are in contact with laminin, stained positively for alpha 6 and beta 4. The entire surface of epithelial cells of solid tumors from patients with serous ovarian carcinoma stained positively for beta 1, alpha 2, and alpha 3 integrins. In most cases, no intact basement membrane surrounded the tumor nests, and staining for alpha 6 and beta 4 was irregular. When present, the basement membrane stained positively for laminin, and the basal surface of the epithelial cells stained positively for alpha 6 and beta 4. Ovarian carcinoma ascites cells exhibited a distinct phenotype, with a significant decrease in expression of the alpha 6 and beta 4 integrin subunits. As alpha 6 and beta 4 integrin subunits are present at the basal surface of many epithelial cells and serve as receptors for laminin, it is possible that ovarian carcinoma epithelial cells may be released from the basement membrane of the ovary due to their deficit of alpha 6 and beta 4 integrin subunits.