Overlap of Viviparous1 (VP1) and abscisic acid response elements in the Em promoter: G-box elements are sufficient but not necessary for VP1 transactivation.
Overlap of Viviparous1 (VP1) and abscisic acid response elements in the Em promoter: G-box elements are sufficient but not necessary for VP1 transactivation.
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DOI：
10.1105/tpc.7.9.1511
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发表时间：
1995-09
期刊：
影响因子：
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通讯作者：
V. Vasil;W. R. Marcotte;Leonard Rosenkrans;S. M. Cocciolone;I. Vasil;Ralph S. Quatrano;Donald R. McCarty
中科院分区：
文献类型：
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作者：
V. Vasil;W. R. Marcotte;Leonard Rosenkrans;S. M. Cocciolone;I. Vasil;Ralph S. Quatrano;Donald R. McCarty
利用缺失分析和定向突变技术研究了小麦Em启动子中介导Viviparous 1（VP 1）反式激活的启动子序列与脱落酸（阿坝）调控的关系。Em 1a的G盒是强烈耦合到VP 1的反式激活以及阿坝的调节，然而，EM启动子包括额外的组件，可以支持VP 1的反式激活没有阿坝的响应或协同作用。几个G-box序列的寡核苷酸四聚体，包括Em 1a，Em 1b，和来自UV光调节的欧芹查尔酮合酶基因的二联体G-box元件，足以赋予VP 1反式激活和与阿坝的协同相互作用到-45花椰菜花叶病毒35 S核心启动子。这些数据表明，VP 1可以通过至少两类顺式作用序列激活转录，包括在C1启动子中发现的G-box元件和Sph调控基序。Em和C1启动子中这些基序的对比作用表明了VP 1对相应基因的差异调节的基础。
The relationship between promoter sequences that mediate Viviparous1 (VP1) transactivation and regulation by abscisic acid (ABA) in the wheat Em promoter was investigated using deletion analysis and directed mutagenesis. The Em1a G-box is strongly coupled to VP1 transactivation as well as to ABA regulation; however, the Em promoter includes additional components that can support VP1 transactivation without ABA responsiveness or synergism. Oligonucleotide tetramers of several G-box sequences, including Em1a, Em1b, and the dyad G-box element from the UV light-regulated parsley chalcone synthase gene, were sufficient to confer VP1 transactivation and the synergistic interaction with ABA to the -45 cauliflower mosaic virus 35S core promoter. These data suggest that VP1 can activate transcription through at least two classes of cis-acting sequences, including the G-box elements and the Sph regulatory motif found in the C1 promoter. The contrasting roles of these motifs in the Em and C1 promoters suggest a basis for the differential regulation of the corresponding genes by VP1.