Glutamate is involved in acid stress response in Bradyrhizobium sp SEMIA 6144 (Arachis hypogaea L.) microsymbiont
Glutamate is involved in acid stress response in Bradyrhizobium sp SEMIA 6144 (Arachis hypogaea L.) microsymbiont
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DOI：
10.1007/s00284-006-0146-y
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发表时间：
2006-12-01
影响因子：
2.6
通讯作者：
Castro, S.
中科院分区：
文献类型：
--
作者：
Natera, V.;Sobrevals, L.;Castro, S.
本文研究了酸胁迫对慢生根瘤菌氨氮同化的影响。Semia 6144(Arachim lowgaea L.)对微生物进行了分析。该菌在酸性pH(5.5)条件下生长，细菌生长速度下降50%，胞内谷氨酸浓度显著升高。谷氨酸合成相关酶的分析表明，在酸胁迫条件下，谷氨酰胺合成酶(GS)和谷氨酸合成酶(NADPH-GOGAT)的活性增加。这支持了GS/NADPH-GOGAT途径有助于增加谷氨酸合成作为酸胁迫反应的相容溶质的观点。
In the present study, the effect of acid stress on ammonium assimilation in Bradyrhizobium sp. SEMIA 6144 (Arachis hypogaea L.) microsymbiont was analyzed. The bacterial growth rate was decreased by 50%, and a significant increase in intracellular glutamate concentration was detected when the strain grew at acid pH (5.5). Assays of the enzymes involved in glutamate synthesis showed increased activities of glutamine synthetase (GS) and glutamate synthase (NADPH-GOGAT) under acid stress condition. This would support the contention that the GS/NADPH-GOGAT pathway contributes to the increase of glutamate synthesis as a compatible solute in response to acid stress.