Expression of Wnt-1 in PC12 cells results in modulation of plakoglobin and E-cadherin and increased cellular adhesion.
Expression of Wnt-1 in PC12 cells results in modulation of plakoglobin and E-cadherin and increased cellular adhesion.
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DOI：
10.1083/jcb.123.6.1857
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发表时间：
1993-12
期刊：
影响因子：
--
通讯作者：
Brown AM
中科院分区：
文献类型：
--
作者：
Bradley RS;Cowin P;Brown AM
WNT-1基因在胎儿大脑发育中起着重要作用，它编码一种分泌蛋白，其信号转导机制目前尚不清楚。在这份报告中，我们研究了Wnt-1基因诱导PC12嗜铬细胞瘤细胞形态变化的细胞内机制。表达WNT-1的PC12细胞显示黏附连接蛋白蛋白的稳态水平增加，并改变了该蛋白在细胞内的分布。这一效应似乎类似于对果蝇胚胎中的白蛋白同系物Armadillo的调制，该同系物对Wnt-1同源物，无翼(Riggleman，B.，P.Schedl和E.Wieschaus)的反应。1990年。牢房。63：549-560)。此外，PC12/WNT-1细胞的E-钙粘附素表达增加，钙依赖的细胞-细胞黏附增加。这些结果意味着细胞对Wnt-1/Wingless的反应在进化上是保守的，并表明在某些细胞类型中，Wnt-1可能起到调节细胞黏附机制的作用。
The Wnt-1 gene plays an essential role in fetal brain development and encodes a secreted protein whose signaling mechanism is presently unknown. In this report we have investigated intracellular mechanisms by which the Wnt-1 gene induces morphological changes in PC12 pheochromocytoma cells. PC12 cells expressing Wnt-1 show increased steady-state levels of the adhesive junction protein plakoglobin, and an altered distribution of this protein within the cell. This effect appears similar to a modulation of the plakoglobin homolog, Armadillo, that occurs in Drosophila embryos in response to the Wnt-1 homolog, wingless (Riggleman, B., P. Schedl, and E. Wieschaus. 1990. Cell. 63:549-560). In addition, PC12/Wnt-1 cells show elevated expression of E-cadherin and increased calcium-dependent cell-cell adhesion. These results imply evolutionary conservation of cellular responses to Wnt- 1/wingless and indicate that in certain cell types Wnt-1 may act to modulate cell adhesion mechanisms.