Optical coherence tomography and fluorescence microscopy dual-modality imaging for in vivo single-cell tracking with nanowire lasers.
Optical coherence tomography and fluorescence microscopy dual-modality imaging for in vivo single-cell tracking with nanowire lasers.
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DOI：
10.1364/boe.395369
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发表时间：
2020-06
影响因子：
3.4
通讯作者：
Xuzhou Li;Wei Zhang;William Y. Wang;Xiaoqin Wu;Yanxiu Li;Xiaotian Tan;Daniel L. Matera;Brendon M. Baker;Y. Paulus;Xudong Fan;Xueding Wang
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文献类型：
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作者：
Xuzhou Li;Wei Zhang;William Y. Wang;Xiaoqin Wu;Yanxiu Li;Xiaotian Tan;Daniel L. Matera;Brendon M. Baker;Y. Paulus;Xudong Fan;Xueding Wang
新兴的基于细胞的治疗，如干细胞治疗和免疫治疗，在生物学研究和临床实践中引起了广泛的关注。然而，基于细胞的疗法的长期技术差距是难以通过跟踪治疗性施用的细胞来直接评估治疗功效。因此，非常需要成像技术来跟踪细胞的体内分布和迁移。光学相干断层扫描（OCT）是一种临床可用的成像技术，具有超高分辨率和出色的成像深度。它也显示出在体内细胞成像的巨大潜力。然而，由于目前OCT细胞标记造影剂（如金和聚合物纳米颗粒）的均匀性，只能观察到整个细胞群的分布。使用这种常规造影剂不可能精确跟踪单个细胞的轨迹。鉴于微激光器尺寸小、发射强度高、发射光谱丰富和线宽窄，微激光器可以提供一种跟踪独特细胞标识符的途径。在这里，我们证明了细胞内化的纳米线激光器提供OCT和荧光信号。此外，细胞可以通过它们携带的纳米线的独特激光发射光谱单独识别。此外，单细胞迁移轨迹可以监测在体外和体内与OCT和荧光显微镜双模态成像系统。我们的研究证明了纳米线激光器与双模态成像系统相结合的可行性，用于具有高空间分辨率和身份验证的体内单细胞跟踪，这是一种对干细胞和免疫调节治疗具有巨大实用性的方法。
Emerging cell-based therapies such as stem cell therapy and immunotherapy have attracted broad attention in both biological research and clinical practice. However, a long-standing technical gap of cell-based therapies is the difficulty of directly assessing treatment efficacy via tracking therapeutically administered cells. Therefore, imaging techniques to follow the in vivo distribution and migration of cells are greatly needed. Optical coherence tomography (OCT) is a clinically available imaging technology with ultrahigh-resolution and excellent imaging depth. It also shows great potential for in vivo cellular imaging. However, due to the homogeneity of current OCT cell labeling contrast agents (such as gold and polymer nanoparticles), only the distribution of entire cell populations can be observed. Precise tracking of the trajectory of individual single cells is not possible with such conventional contrast agents. Microlasers may provide a route to track unique cell identifiers given their small size, high emission intensities, rich emission spectra, and narrow linewidths. Here, we demonstrate that nanowire lasers internalized by cells provide both OCT and fluorescence signal. In addition, cells can be individually identified by the unique lasing emission spectra of the nanowires that they carry. Furthermore, single cell migration trajectories can be monitored both in vitro and in vivo with OCT and fluorescence microscopy dual-modality imaging system. Our study demonstrates the feasibility of nanowire lasers combined with the dual-modality imaging system for in vivo single cell tracking with a high spatial resolution and identity verification, an approach with great utility for stem cell and immunomodulatory therapies.