Elevated CD14++CD16+ Monocytes in Hyperhomocysteinemia-Associated Insulin Resistance in Polycystic Ovary Syndrome
Elevated CD14++CD16+ Monocytes in Hyperhomocysteinemia-Associated Insulin Resistance in Polycystic Ovary Syndrome
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多囊卵巢综合征中高同型半胱氨酸血症相关的胰岛素抵抗中 CD14( )CD16( ) 单核细胞升高
DOI：
10.1177/1933719118756772
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发表时间：
2018-12-01
影响因子：
2.9
通讯作者：
Qiao, Jie
中科院分区：
文献类型：
--
作者：
Zhang, Bochun;Qi, Xinyu;Qiao, Jie
背景资料：高同型半胱氨酸血症、慢性低度炎症和胰岛素抵抗是多囊卵巢综合征（PCOS）女性的主要特征。CD 14（++）CD 16（+）炎性单核细胞在心血管疾病和糖尿病中升高，炎症是其发病机制之一。我们的目的是确定同型半胱氨酸水平是否与多囊卵巢综合征患者的单核细胞亚型或胰岛素抵抗有关。方法：2014年12月至2015年6月在北京大学第三医院进行横断面研究。在196例中国PCOS患者中，102例同型半胱氨酸水平>= 10 μ mol/L，94例同型半胱氨酸水平正常。采用流式细胞术检测外周血单核细胞表面标志物和相关细胞因子，并收集胰岛素抵抗和同型半胱氨酸水平的数据。结果如下：我们的研究结果显示，高同型半胱氨酸血症PCOS组的空腹胰岛素和反映胰岛素抵抗严重程度的稳态模型评估指标增加。一元线性回归分析显示，同型半胱氨酸水平是PCOS患者胰岛素抵抗的影响因素之一。与同型半胱氨酸正常组相比，高同型半胱氨酸血症患者外周血中CD 14（++）CD 16（+）炎性单核细胞数量增加，血浆中炎性单核细胞分泌的IL-1 β和IL-6水平升高。与CD 14（+）CD 16（++）非经典单核细胞不同，CD 14（++）CD 16（+）炎性单核细胞以高表达人类白细胞抗原-抗原D相关（HLA-DR）为特征。结论：我们的研究结果表明CD 14（++）CD 16（+）炎症单核细胞与多囊卵巢综合征患者的高同型半胱氨酸血症和胰岛素抵抗有关。值得注意的是，CD 14（++）CD 16（+）单核细胞可能有助于PCOS妇女胰岛素抵抗的发病机制。
Background: Hyperhomocysteinemia, chronic low-grade inflammation, and insulin resistance are predominant features in women with polycystic ovary syndrome (PCOS). CD14(++)CD16(+) inflammatory monocytes are elevated in cardiovascular diseases and diabetes, in which inflammation is one of the pathogenesis. We aimed to determine whether homocysteine levels are associated with monocyte subtypes or insulin resistance in women with PCOS. Methods: A cross-sectional study was conducted between December 2014 and June 2015 at Peking University Third Hospital. Among 196 Chinese patients with PCOS enrolled, 102 had homocysteine levels >= 10 mu mol/L and 94 exhibited normal homocysteine levels. Monocyte surface markers and related cytokines were detected in peripheral blood samples using a flow cytometry, and data on insulin resistance and homocysteine levels were collected. Results: Our results showed that fasting insulin and homeostasis model assessment indices that reflect the severity of insulin resistance were increased in the hyperhomocysteinemia PCOS group. Simple linear regression analysis revealed that homocysteine level is one of the influence factors of insulin resistance in PCOS. Compared with the normal homocysteine group, patients with hyperhomocysteinemia had increased numbers of CD14(++)CD16(+) inflammatory monocyte in their peripheral blood and elevated plasma levels of interleukin-1 beta and interleukin-6, 2 typical cytokines secreted by the inflammatory monocytes. Unlike CD14(+)CD16(++) nonclassical monocytes, CD14(++)CD16(+) inflammatory monocytes are characterized by high expression of Human leukocyte antigen-antigen D related (HLA-DR). Conclusions: Our findings indicate that CD14(++)CD16(+) inflammatory monocytes are associated with hyperhomocysteinemia and insulin resistance in patients with PCOS. Notably, CD14(++)CD16(+) monocytes may contribute to the pathogenesis of insulin resistance in women with PCOS.