IFI16 and NM23 Bind to a Common DNA Fragment Both in the P53 and the cMYC Gene Promoters
IFI16 and NM23 Bind to a Common DNA Fragment Both in the P53 and the cMYC Gene Promoters
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DOI：
10.1002/jcb.22053
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发表时间：
2009-03-01
影响因子：
4
通讯作者：
Cervoni, Laura
中科院分区：
文献类型：
--
作者：
Egistelli, Lorenza;Chichiarelli, Silvia;Cervoni, Laura
在黑色素瘤M14细胞系中，我们发现抗转移蛋白NM 23/核苷二磷酸激酶与癌基因cMYC和P53的启动子结合，P53是一种在人类癌症中经常突变的基因（Cervoni等人[2006] J. Cell. 98：421-428）。在进一步的研究中，我们现在发现，IFI 16，一个转录抑制因子，在两个启动子中结合到富含G的片段，该片段也结合NM 23/NDPK。这些片段具有非B DNA结构。此外，通过连续染色质免疫沉淀（re-ChIP），我们发现这两种蛋白质（IFI 16和NM 23/NDPK）在体内同时结合到相同的DNA片段。由于P53刺激细胞凋亡并抑制细胞生长，而cMYC促进细胞生长，在某些情况下还促进细胞凋亡，因此在相同DNA片段上存在NM 23和IFI 16表明它们共同参与了某些肿瘤的发展。J.细胞。106：666-672，2009. (C)2009 Wiley-Liss，Inc.
In the melanoma M14 cell line, we found that the antimetastatic protein NM23/nucleoside diphosphate kinase binds to the promoters Of the oncogene cMYC and of P53, a gene often mutated in human cancer(Cervoni et al. [2006] J. Cell. Biochem. 98:421-428). In a further study, we find now that IFI16, a transcriptional repressor, in both promoters binds to the G-rich fragment that also binds NM23/NDPK. These fragments possess non-B DNA structures. Moreover, by sequential chromatin immunoprecipitation (re-ChIP) we show that the two proteins (IFI16 and NM23/NDPK) are simultaneously bound in vivo to the same DNA fragments. Since P53 stimulates apoptosis and inhibits cellular growth, and cMYC promotes cell growth and, in several instances, also apoptosis, the presence of NM23 and IFI16 on the same DNA fragments suggests their common involvement in the reduced development of some tumors. J. Cell. Biochem. 106: 666-672, 2009. (C) 2009 Wiley-Liss, Inc.