Overexpression of rice serotonin N-acetyltransferase 1 in transgenic rice plants confers resistance to cadmium and senescence and increases grain yield
Overexpression of rice serotonin N-acetyltransferase 1 in transgenic rice plants confers resistance to cadmium and senescence and increases grain yield
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DOI：
10.1111/jpi.12392
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发表时间：
2017-04-01
影响因子：
10.3
通讯作者：
Back, Kyoungwhan
中科院分区：
文献类型：
--
作者：
Lee, Kyungjin;Back, Kyoungwhan
虽然5 -羟色胺n -乙酰转移酶（SNAT）在植物中的异位过表达已经通过动物SNAT基因实现，但植物SNAT基因在植物中的异位过表达尚未被研究。由于植物SNAT蛋白在亚细胞定位和酶动力学上与动物不同，其在植物中的异位过表达可能会产生与转基因植物中动物SNAT基因过表达所观察到的结果不同的结果。与我们的预期一致，我们发现过表达水稻（Oryza sativa） SNAT1 （OsSNAT1）的转基因水稻植株没有表现出像在过表达snat的转基因水稻植株中观察到的幼苗生长增强，尽管两种类型的植株都表现出增加的褪黑激素水平。与野生型对照相比，ossnat1过表达水稻确实表现出对镉和衰老胁迫的显著抗性。与番茄不同，水稻幼苗褪黑素合成不受硒诱导，OsSNAT1转基因水稻植株对硒不具有耐受性。在水田条件下，T-2纯合子OsSNAT1转基因水稻由于单株穗数增加而表现出产量的增加。OsSNAT1异位过表达所带来的这些益处在过表达绵羊SNAT的转基因水稻中未被观察到，这表明植物SNAT的功能与动物SNAT不同。
While ectopic overexpression of serotonin N-acetyltransferase (SNAT) in plants has been accomplished using animal SNAT genes, ectopic overexpression of plant SNAT genes in plants has not been investigated. Because the plant SNAT protein differs from that of animals in its subcellular localization and enzyme kinetics, its ectopic overexpression in plants would be expected to give outcomes distinct from those observed from overexpression of animal SNAT genes in transgenic plants. Consistent with our expectations, we found that transgenic rice plants overexpressing rice (Oryza sativa) SNAT1 (OsSNAT1) did not show enhanced seedling growth like that observed in ovine SNAT-overexpressing transgenic rice plants, although both types of plants exhibited increased melatonin levels. OsSNAT1-overexpressing rice plants did show significant resistance to cadmium and senescence stresses relative to wild-type controls. In contrast to tomato, melatonin synthesis in rice seedlings was not induced by selenium and OsSNAT1 transgenic rice plants did not show tolerance to selenium. T-2 homozygous OsSNAT1 transgenic rice plants exhibited increased grain yield due to increased panicle number per plant under paddy field conditions. These benefits conferred by ectopic overexpression of OsSNAT1 had not been observed in transgenic rice plants overexpressing ovine SNAT, suggesting that plant SNAT functions differently from animal SNAT in plants.