Site-directed and random mutagenesis in Porphyromonas gingivalis: Construction of fimbriae-related-gene mutant
Site-directed and random mutagenesis in Porphyromonas gingivalis: Construction of fimbriae-related-gene mutant
复制标题
牙龈卟啉单胞菌的定点和随机诱变：菌毛相关基因突变体的构建
DOI：
10.1007/978-1-0716-0939-2_1
复制
发表时间：
2021
影响因子：
--
通讯作者：
and Nagano K.
中科院分区：
文献类型：
--
作者：
Nishiyama S.; Hasegawa;Y.;and Nagano K.
牙龈卟啉单胞菌（Porphyromonasgingivalis）是一种革兰氏阴性球杆菌，是引起慢性牙周炎的病原菌。遗传学方法极大地促进了在分子水平上对生物体的研究。虽然存在一些挑战，但利用遗传技术（如构建敲除突变体）在P。牙龈炎是可行的。在本章中，我们描述了通过构建菌毛相关基因突变体的定点突变和随机突变的详细方法。牙龈炎
Porphyromonas gingivalis, an etiological agent of chronic periodontitis, is an asaccharolytic anaerobic gram-negative coccobacillus. Genetic approaches greatly facilitate research on organisms at the molecular level. Although with some challenges, the use of genetic techniques (such as constructing knockout mutants) inP. gingivalisare feasible. In this chapter, we describe detailed methods for site-directed and random mutagenesis through the construction of fimbriae-related gene mutants ofP. gingivalis.