The impact of rearing environment on the development of gut microbiota in tilapia larvae.
The impact of rearing environment on the development of gut microbiota in tilapia larvae.
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DOI：
10.1038/srep18206
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发表时间：
2015-12-11
影响因子：
4.6
通讯作者：
Verdegem M
中科院分区：
文献类型：
--
作者：
Giatsis C;Sipkema D;Smidt H;Heilig H;Benvenuti G;Verreth J;Verdegem M
本研究探讨了饲养环境对尼罗罗非鱼（Oreochromis niloticus, Linnaeus）幼虫在循环或主动悬浮系统中生长的水中细菌群落（BC）的影响及其与肠道细菌群落的关系。对pcr扩增的16S rRNA基因片段进行454焦磷酸测序，以表征水、饲料和肠道细菌群落的组成。观察到的水BC随时间的变化以及不同系统间水BC的差异与相应的水物理化学性质高度相关。幼虫发育过程中肠道细菌群落的差异与不同系统间水群落的差异有关。与肠道和水之间的相关性相比，饲料BC与肠道中的相关性较小，这反映在水和肠道之间共享的otu是肠道和饲料BC之间共享的otu的4至43倍。水和肠道之间共享的otu表明微生物成功地从水中转移到肠道，并深入了解肠道内水微生物的生态位和生态适应性。这些发现表明，通过饲养系统的设计和功能衍生的水微生物管理可以控制肠道微生物群落。
This study explores the effect of rearing environment on water bacterial communities (BC) and the association with those present in the gut of Nile tilapia larvae (Oreochromis niloticus, Linnaeus) grown in either recirculating or active suspension systems. 454 pyrosequencing of PCR-amplified 16S rRNA gene fragments was applied to characterize the composition of water, feed and gut bacteria communities. Observed changes in water BC over time and differences in water BCs between systems were highly correlated with corresponding water physico-chemical properties. Differences in gut bacterial communities during larval development were correlated with differences in water communities between systems. The correlation of feed BC with those in the gut was minor compared to that between gut and water, reflected by the fact that 4 to 43 times more OTUs were shared between water and gut than between gut and feed BC. Shared OTUs between water and gut suggest a successful transfer of microorganisms from water into the gut, and give insight about the niche and ecological adaptability of water microorganisms inside the gut. These findings suggest that steering of gut microbial communities could be possible through water microbial management derived by the design and functionality of the rearing system.