Partial purification and characterization of mammary stimulating factor, a protein which promotes proliferation of mammary epithelium.
Partial purification and characterization of mammary stimulating factor, a protein which promotes proliferation of mammary epithelium.
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乳腺刺激因子的部分纯化和表征，乳腺刺激因子是一种促进乳腺上皮增殖的蛋白质。
DOI：
10.1021/bi00583a015
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发表时间：
1979
期刊：
影响因子：
2.9
通讯作者：
F. Stockdale
中科院分区：
文献类型：
--
作者：
K. Ptashne;H. Hsueh;F. Stockdale
本报告描述了乳腺刺激因子（MSF）的部分纯化和特性，MSF是一种从血清中分离的有丝分裂肽，可启动小鼠乳腺上皮的生长。通过离子交换层析、凝胶过滤和等电聚焦，从猪血清中纯化了250倍的MSF。它是一种热稳定的蛋白质，分子量为10,100—10,400，等电点为5.5—6.0。与小鼠乳腺肿瘤细胞（CZF）、3T3细胞或鸡胚胎细胞相比，MSF在体外促进乳腺上皮细胞DNA合成的程度更大。将MSF与其他已建立的促生长肽的生物学、物理和免疫学特性进行比较，表明MSF是一种独特的血清因子。
This report describes the partial purification and characterization of mammary stimulating factor (MSF), a mitogenic peptide isolated from serum which initiates growth in mouse mammary epithelium. By using ion-exchange chromatography, gel filtration, and isoelectric focusing, MSF was purified 250-fold from porcine serum. It is a heat-stable protein of molecular weight 10,100--10,400 with an isoelectric point of 5.5--6.0. MSF initiates DNA synthesis in vitro in mammary epithelium to a greater extent than in mouse mammary tumor cells (CZF), 3T3 cells, or chick embryo cells. Comparison of the biological, physical, and immunological properties of MSF with other established growth-promoting peptides suggests that MSF is a unique serum factor.