Genes involved in the synthesis and degradation of matrix polysaccharide in Actinobacillus actinomycetemcomitans and Actinobacillus pleuropneumoniae biofilms
Genes involved in the synthesis and degradation of matrix polysaccharide in Actinobacillus actinomycetemcomitans and Actinobacillus pleuropneumoniae biofilms
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DOI：
10.1128/jb.186.24.8213-8220.2004
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发表时间：
2004-12-01
影响因子：
3.2
通讯作者：
Ramasubbu, N
中科院分区：
文献类型：
--
作者：
Kaplan, JB;Velliyagounder, K;Ramasubbu, N
生物膜由包埋在胞外多糖基质中的细菌细胞组成。大肠杆菌生物膜基质的主要成分是PGA，一种β（1，6）键N-乙酰基-D-葡糖胺残基的线性聚合物。PGA介导细胞间粘附和细胞对非生物表面的附着。在这份报告中，我们提出的遗传和生化证据表明，PGA也是一个主要的基质成分的生物膜所产生的人类牙周病原体放线菌放线杆菌和猪呼吸道病原体胸膜肺炎放线杆菌。我们还发现PGA是分散素B的底物，分散素B是由A.放线菌共生菌，并且正链菌分散素B酶由放线菌共生菌产生。胸膜肺炎我们进一步证明了A.伴随放线菌PGA与抗多糖细胞间粘附素的抗血清发生交叉反应，多糖细胞间粘附素是一种与PGA在遗传和结构上相关的葡萄球菌生物膜基质多糖。我们的研究结果证实，PGA功能作为生物膜基质多糖在不同的细菌物种，并建议PGA可能发挥作用，在细胞间粘附和细胞分离和分散在A。伴随放线菌和A.胸膜肺炎生物膜
Biofilms are composed of bacterial cells embedded in an extracellular polysaccharide matrix. A major component of the Escherichia coli biofilm matrix is PGA, a linear polymer of N-acetyl-D-glucosamine residues in beta(1,6) linkage. PGA mediates intercellular adhesion and attachment of cells to abiotic surfaces. In this report, we present genetic and biochemical evidence that PGA is also a major matrix component of biofilms produced by the human periodontopathogen Actinobacillus actinomycetemcomitans and the porcine respiratory pathogen Actinobacillus pleuropneumoniae. We also show that PGA is a substrate for dispersin B, a biofilm-releasing glycosyl hydrolase produced by A. actinomycetemcomitans, and that an orthologous dispersin B enzyme is produced by A. pleuropneumoniae. We further show that A. actinomycetemcomitans PGA cross-reacts with antiserum raised against polysaccharide intercellular adhesin, a staphylococcal biofilm matrix polysaccharide that is genetically and structurally related to PGA. Our findings confirm that PGA functions as a biofilm matrix polysaccharide in phylogenetically diverse bacterial species and suggest that PGA may play a role in intercellular adhesion and cellular detachment and dispersal in A. actinomycetemcomitans and A. pleuropneumoniae biofilms.