Effects of radiofrequency exposure on the GABAergic system in the rat cerebellum: clues from semi-quantitative immunohistochemistry
Effects of radiofrequency exposure on the GABAergic system in the rat cerebellum: clues from semi-quantitative immunohistochemistry
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DOI：
10.1016/s0006-8993(01)02599-9
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发表时间：
2001-08-31
期刊：
影响因子：
2.9
通讯作者：
Privat, A
中科院分区：
文献类型：
--
作者：
Mausset, AL;de Seze, R;Privat, A
蜂窝电话的广泛使用引发了电磁场与中枢神经系统（CNS）相互作用的问题。为了测量这些对中枢神经系统神经递质含量的影响，我们开发了一种基于免疫组织化学和图像分析的神经递质检测方案。 γ-乙烯基-GABA (GVG) 是一种 GABA 转氨酶抑制剂，被注射到大鼠体内以增加 CNS 中的 GABA 浓度。然后通过免疫组织化学揭示细胞 GABA 含量，并通过图像分析根据三个参数进行半定量：光密度 (O.D.)、染色面积和阳性细胞数量。 GVG 1200 mg/kg 诱导的小脑 GABA 含量增加反映在分子层和颗粒层的这三个参数中。因此，免疫组织化学参数的控制以及适当的图像分析可以定位和检测细胞神经递质含量的变化。该协议用于研究暴露于 900 MHz 射频对小脑 GABA 含量的影响。测试了比吸收率 (SAR) 为 4 W/kg 的脉冲发射和高 SAR (32 W/kg) 的连续发射。我们观察到暴露于脉冲射频后，浦肯野细胞层中的染色过程区域选择性减少，此外，O.D. 也减少。暴露于连续波后的三个细胞层中。目前尚不清楚这种效应是否至少部分是由于组织的局部加热造成的。总体而言，高能射频暴露似乎会导致小脑细胞 GABA 含量减少。 (C) 2001 Elsevier Science B.V. 保留所有权利。
The widespread use of cellular phones raises the problem of interaction of electromagnetic fields with the central nervous system (CNS). In order to measure these effects on neurotransmitter content in the CNS, we developed a protocol of neurotransmitter detection based on immunohistochemistry and image analysis. Gamma-vinyl-GABA (GVG), an inhibitor of the GABA-transaminase was injected in rats to increase GABA concentration in the CNS. The cellular GABA contents were then revealed by immunohistochemistry and semi-quantified by image analysis thanks to three parameters: optical density (O.D.), staining area, and number of positive cells. The increase in cerebellar GABA content induced by GVG 1200 mg/kg was reflected in these three parameters in the molecular and the granular layers. Therefore, control of immunohistochemistry parameters, together with appropriate image analysis, allowed both the location and the detection of variations in cellular neurotransmitter content. This protocol was used to investigate the effects of exposure to 900 MHz radiofrequencies on cerebellar GABA content. Both pulsed emission with a specific absorption rate (SAR) of 4 W/kg and continuous emission with high SAR (32 W/kg) were tested. We observed a selective diminution of the stained processes area in the Purkinje cell layer after exposure to pulsed radiofrequency and, in addition, a decrease in O.D. in the three cell layers after exposure to continuous waves. Whether this effect is, at least partly, due to a local heating of the tissues is not known. Overall, it appears that high energetic radiofrequency exposure induces a diminution in cellular GABA content in the cerebellum. (C) 2001 Elsevier Science B.V. All rights reserved.