A Taxonomic Study of Thermomonospora and Other Monosporic Actinomycetes
A Taxonomic Study of Thermomonospora and Other Monosporic Actinomycetes
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DOI：
10.1099/00221287-130-1-5
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发表时间：
1984
期刊：
影响因子：
1.5
通讯作者：
A. Mccarthy;T. Cross
中科院分区：
文献类型：
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作者：
A. Mccarthy;T. Cross
总结：大量的热单孢菌属分离株和典型或参考培养物，以及热放线菌属、热单孢菌属、小多孢菌属和马杜拉放线菌属的代表，被纳入数值分类研究。对113个供试菌株的101个形态、生理生化性状进行了测定，并采用简单匹配（SSM）、Jaccard（SJ）和模式差异（DP）系数对数据进行了分析，同时采用单对聚类法和未加权对群平均聚类法对数据进行了聚类分析。所有的分析都产生了相似的分类，受测试误差的影响很小，估计为1.1%。热单孢菌菌株形成两个主要的集群，被鉴定为“Thm.产色菌”和白色热单孢菌群，而“Acm. flexuosa”，Mip. faeni和Thm. mesophila很好地彼此分离，所有主要的集群。两个Thermomonospora簇之间的低表型相似性支持基于化学数据的建议，即“Thm.“产色菌”可能更好地分类在一个单独的属。在白色热单孢菌群中检测到三个亚群，并与物种“Thm.”等同。fusca”，Thm. curvata和Thm.阿尔巴。该菌群是异质的，尽管它包括被鉴定为Sam的菌株。viridis，“Mip.（丁香），不能区分种。模式菌株“Thm.“galeriensis”具有该菌群的显著特征，应被视为单孢菌属的一员。只有5株Thermoactinomyces被包括在内，但他们形成了一个集群的内部结构，支持以前的数量分类的物种在本属。壁化学型和孢子耐热性与属鉴定一致。作为该研究的结果，五个Thermomonospora spp.他说：“Thm。nov. nom. rev.（同义词“Actinobifida chromogena”，“Thm. falcata”）; Thm. mesophila;“Thm. fusca”sp. nov. nom. rev.; Thm. alba（同义词Thm. mesouviformis）; Thm.弯曲的实验数据允许这些物种的鉴定方案的发展，也提供了测试，以确认其他嗜热放线菌菌株的通用标识。
SUMMARY: A large collection of Thermomonospora isolates and type or reference cultures, together with representatives of the genera Thermoactinomyces, Saccharomonospora, Micropolyspora and Actinomadura, were included in a numerical taxonomic study. The 113 strains were examined for 101 morphological, physiological and biochemical characters and data were analysed using simple matching (SSM ), Jaccard (SJ ) and pattern difference (DP ) coefficients with clustering by both single and unweighted pair group average algorithms. All of the analyses produced similar classifications which were little affected by test error, estimated at 1.1%. Thermomonospora strains formed two major clusters identified as “Thm. chromogena” and the white Thermomonospora group, respectively, while type strains of “Acm. flexuosa”, Mip. faeni and Thm. mesophila were well separated from one another and all major clusters. The low phenetic similarity between the two Thermomonospora clusters supports suggestions based on chemical data that “Thm. chromogena” may be better classified in a separate genus. Three subclusters were detected within the white Thermomonospora group and equated with the species “Thm. fusca”, Thm. curvata and Thm. alba. The Saccharomonospora cluster was heterogeneous and, although it included strains identified as Sam. viridis, “Mip. caesia” and Thermomonospora sp. (lilac), species could not be distinguished. The type strain of “Thm. galeriensis” shared the distinguishing characteristics of this cluster and should be regarded as a member of the genus Saccharomonospora. Only five strains of Thermoactinomyces were included, but they formed a cluster with an internal structure which supported a previous numerical classification of species in this genus. Wall chemotype and spore heat-resistance properties were consistent with generic identification. As a result of this study, five Thermomonospora spp. are recognized: “Thm. chromogena” comb. nov. nom. rev. (synonyms “Actinobifida chromogena”, “Thm. falcata”); Thm. mesophila; “Thm. fusca” sp. nov. nom. rev.; Thm. alba (synonym Thm. mesouviformis); Thm. curvata. The experimental data allowed the development of an identification scheme for these species and also provided tests to confirm generic identifications of other thermophilic actinomycete isolates.