Application of a PDMS microstencil as a replaceable insulator toward a single-use planar microelectrode array.
Application of a PDMS microstencil as a replaceable insulator toward a single-use planar microelectrode array.
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应用 PDMS 微模板作为一次性平面微电极阵列的可更换绝缘体。
DOI：
10.1007/s10544-006-9145-9
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发表时间：
2006
影响因子：
2.8
通讯作者：
Wheeler,BruceC
中科院分区：
文献类型：
--
作者：
Nam,Yoonkey;Musick,Katherine;Wheeler,BruceC
在这里，我们提出了一种可更换绝缘体的新想法，从而朝着用于生电组织研究的一次性平面微电极阵列（MEA）的目标迈进。可更换绝缘体的概念是由于重复使用 MEA 后绝缘体退化而提出的。我们建议更换绝缘体并为每次实验有效地生产新的 MEA，而不是制造更耐用的绝缘体来重复使用 MEA。我们选择聚二甲基硅氧烷 (PDMS) 微模板作为可更换绝缘体的候选材料，因为它具有生物相容性，对表面具有可逆粘附力，并且可以轻松、可控地制造。作为概念验证，我们展示了使用微模板的两种应用：旧 MEA 的复兴和一次性 MEA 的制造。这些 MEA 使用分离的神经细胞培养物进行了测试，并在 14 天时进行了体外神经记录。具有微米尺寸孔的绝缘体的廉价且快速的供应提供了一种构建 MEA 的方法，该 MEA 可被视为高通量基于细胞的生物传感器应用中的一次性组件。
Here we present a novel idea for a replaceable insulator, and thus advance toward the goal of a single-use planar microelectrode array (MEA) for the study of electrogenic tissues. The concept of a replaceable insulator is motivated by insulator degradation after repeated usage of an MEA. Instead of fabricating a more durable insulator for repeated MEA usage, we propose replacing the insulator and effectively producing a fresh MEA for each experiment. We chose a polydimethylsiloxane (PDMS) microstencil as a candidate for the replaceable insulator as it is biocompatible, shows reversible adhesion to surfaces, and can be easily and controllably fabricated. As a proof-of-concept, we demonstrate two applications using microstencils: the rejuvenation of an old MEA and the fabrication of a single-use MEA. These MEAs were tested with dissociated neural cell cultures and neural recordings were performed at 14 daysin vitro. Inexpensive and quick supply of insulators with micrometer-sized holes provides a way of constructing an MEA that can be treated as a disposable component in high throughput cell-based biosensor applications.