Fat accretion measurements strengthen the relationship between feed conversion efficiency and Nitrogen isotopic discrimination while rumen microbial genes contribute little.
Fat accretion measurements strengthen the relationship between feed conversion efficiency and Nitrogen isotopic discrimination while rumen microbial genes contribute little.
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DOI：
10.1038/s41598-018-22103-4
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发表时间：
2018-03-01
影响因子：
4.6
通讯作者：
Dewhurst RJ
中科院分区：
文献类型：
--
作者：
Meale SJ;Auffret MD;Watson M;Morgavi DP;Cantalapiedra-Hijar G;Duthie CA;Roehe R;Dewhurst RJ
使用生物标记物来测定饲料转化率（FCE），如氮同位素鉴别（Δ 15 N），有助于更容易的测量，并可用于育种策略。然而，我们需要更好地了解饲料消耗与Δ 15 N之间的关系，特别是增重和瘤胃N代谢组成差异的影响。除了FCE和Δ 15 N的测量外，我们还估计了身体组成的变化，并使用含和不含硝酸盐的饮食处理以及瘤胃宏基因组学来探索这些影响。与提供不含硝酸盐的饮食的牛相比，饲喂硝酸盐的牛具有降低的FCE和较高的血浆中Δ 15 N。FCE与Δ 15 N之间的负相关关系随着第3腰椎脂肪深度变化的增加而增强，但与平均日增重之间的负相关关系不明显。我们鉴定了1,700个相对丰度>0.01%的微生物基因，其中26个与Δ 15 N相关。这些基因解释了Δ 15 N中69%的变异，并在两个不同的功能网络中显示出聚类。但它们的相对丰度与Δ 15 N之间没有明显的相关性，说明瘤胃微生物基因对Δ 15 N的贡献很小。相反，我们发现，增益组成的变化（脂肪增加）为FCE和Δ 15 N之间的关系提供了额外的强度。
The use of biomarkers for feed conversion efficiency (FCE), such as Nitrogen isotopic discrimination (Δ15N), facilitates easier measurement and may be useful in breeding strategies. However, we need to better understand the relationship between FCE and Δ15N, particularly the effects of differences in the composition of liveweight gain and rumen N metabolism. Alongside measurements of FCE and Δ15N, we estimated changes in body composition and used dietary treatments with and without nitrates, and rumen metagenomics to explore these effects. Nitrate fed steers had reduced FCE and higher Δ15N in plasma compared to steers offered non-nitrate containing diets. The negative relationship between FCE and Δ15N was strengthened with the inclusion of fat depth change at the 3rd lumbar vertebrae, but not with average daily gain. We identified 1,700 microbial genes with a relative abundance >0.01% of which, 26 were associated with Δ15N. These genes explained 69% of variation in Δ15N and showed clustering in two distinct functional networks. However, there was no clear relationship between their relative abundances and Δ15N, suggesting that rumen microbial genes contribute little to Δ15N. Conversely, we show that changes in the composition of gain (fat accretion) provide additional strength to the relationship between FCE and Δ15N.
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影响因子：
5.2
作者：
Auffret MD;Stewart R;Dewhurst RJ;Duthie CA;Rooke JA;Wallace RJ;Freeman TC;Snelling TJ;Watson M;Roehe R
通讯作者：
Roehe R
影响因子：
3.3
作者：
Cheng, L.;Sheahan, A. J.;Roche, J. R.
通讯作者：
Roche, J. R.
影响因子：
3.5
作者：
Toral, P. G.;Bernard, L.;Frutos, P.
通讯作者：
Frutos, P.
影响因子：
3.6
作者：
Duthie, C. -A.;Troy, S. M.;Rooke, J. A.
通讯作者：
Rooke, J. A.
影响因子：
2.8
作者：
Arai, H;Mizutani, M;Igarashi, Y
通讯作者：
Igarashi, Y