Identification and characterization of Cor413im proteins as novel components of the chloroplast inner envelope
Identification and characterization of Cor413im proteins as novel components of the chloroplast inner envelope
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DOI：
10.1111/j.1365-3040.2008.01854.x
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发表时间：
2008-10-01
影响因子：
7.3
通讯作者：
Inaba, Takehito
中科院分区：
文献类型：
--
作者：
Okawa, Kumiko;Nakayama, Katsuhiro;Inaba, Takehito
质体被两层膜包围，即外被膜和内被膜，它们具有各种运输和代谢活动。许多包膜蛋白已被确定的生化方法，并已分配到特定的功能。尽管这些努力，叶绿体被膜预计含有一些尚未鉴定的蛋白质，可能会影响植物生长和发育的特定方面。在这份报告中，我们确定和表征一类新的内被膜蛋白，命名为Cor 413叶绿体内被膜组（Cor 413 im）。体内和体外研究均表明Cor 413 im蛋白靶向叶绿体被膜。生化分析表明，Cor 413 im 1是一个完整的膜蛋白在叶绿体的内被膜。实时荧光定量PCR分析表明，在冷驯化的拟南芥叶片中，COR 413 IM 1比COR 413 IM 2更丰富。对T-DNA插入突变体的分析表明，一个拷贝的COR 413 IM基因足以提供拟南芥正常的抗冻性。基于这些数据，我们提出Cor 413 im蛋白是针对叶绿体内被膜响应低温的新组分。
Plastids are surrounded by two membrane layers, the outer and inner envelope membranes, which have various transport and metabolic activities. A number of envelope membrane proteins have been identified by biochemical approaches and have been assigned to specific functions. Despite those efforts, the chloroplast envelope membrane is expected to contain a number of as yet unidentified proteins that may affect specific aspects of plant growth and development. In this report, we identify and characterize a novel class of inner envelope membrane proteins, designated as Cor413 chloroplast inner envelope membrane group (Cor413im). Both in vivo and in vitro studies indicate that Cor413im proteins are targeted to the chloroplast envelope. Biochemical analyses of Cor413im1 demonstrate that it is an integral membrane protein in the inner envelope of chloroplasts. Quantitative real-time PCR analysis reveals that COR413IM1 is more abundant than COR413IM2 in cold-acclimated Arabidopsis leaves. The analyses of T-DNA insertion mutants indicate that a single copy of COR413IM genes is sufficient to provide normal freezing tolerance to Arabidopsis. Based on these data, we propose that Cor413im proteins are novel components that are targeted to the chloroplast inner envelope in response to low temperature.