A proposed model to explain persistent infection of host cells with Coxiella burnetii.
A proposed model to explain persistent infection of host cells with Coxiella burnetii.
复制标题
提出的模型解释伯内氏立克次体对宿主细胞的持续感染。
DOI：
10.1099/00221287-132-5-1415
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Baca,OG
中科院分区：
文献类型：
--
作者：
Roman,MJ;Coriz,PD;Baca,OG
L929小鼠成纤维细胞和J774巨噬细胞样细胞均对Q热病原体贝氏柯克斯体的持续感染敏感。以前，该实验室已经表明，持续感染的细胞群体繁殖的世代时间或细胞周期的进展不变。其他人和我们也报道过，高度感染的细胞通常表现出一个含有寄生虫的大空泡。我们现在报告说，轻度和重度感染的细胞能够分裂，并在此过程中分离出含寄生虫的空泡到一个新兴的子细胞;伴侣子细胞出现寄生虫免费。通过染色细胞的显微摄影显示的感染细胞的这种不对称分裂解释了先前100%感染的持续感染宿主细胞群中未感染细胞的出现。一些持续感染的L929群体在不添加正常细胞的情况下在培养物中维持两年以上。
L929 mouse fibroblast cells and J774 macrophage-like cells are both susceptible to persistent infection with the Q fever agentCoxiella burnetii. Previously this laboratory has shown that persistently infected cell populations multiply with unaltered generation times or cell cycle progression. It has also been reported by others and us that highly infected cells typically exhibit one large parasite-containing vacuole. We now report that lightly and heavily infected cells are capable of division and in the process segregate the parasite-containing vacuole into one of the emerging daughter cells; the companion daughter cell emerges parasite-free. This asymmetric division of infected cells, revealed via photomicrography of stained cells, accounts for the appearance of uninfected cells within persistently infected host cell populations that were previously 100% infected. Some of the persistently infected L929 populations were maintained in culture for over two years without the addition of normal cells.