Time course of gene expression in rat experimental autoimmune myocarditis
Time course of gene expression in rat experimental autoimmune myocarditis
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DOI：
10.1042/cs1030623
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发表时间：
2002-12-01
期刊：
影响因子：
6
通讯作者：
Aizawa, Y
中科院分区：
文献类型：
--
作者：
Hanawa, H;Abe, S;Aizawa, Y
实验性自身免疫性心肌炎（EAM）的遗传反应特征尚未确定。为了研究EAM心肌中的基因表达，在第0天使用实时逆转录酶PCR评估从固定量的总心脏RNA合成的cDNA中的44种mRNA种类[钙处理蛋白、收缩蛋白、利钠肽（NP）、细胞因子、趋化因子、生长因子、肾素-血管紧张素-醛固酮（RAA）系统、内皮素（ET）和细胞外基质]的绝对拷贝数，免疫后14、21和28。在第14天，α-心肌肌球蛋白减少26.3倍，β-心肌肌球蛋白增加3.75倍。在第21天和第14天，心房NP和脑NP分别增加47.7倍和6.35倍。血管紧张素II I型受体、血管紧张素转换酶和ETI在21天分别增加22.3倍、6.30倍和16.8倍。醛固酮受体在第14天下降2.15倍，但醛固酮合成酶仅在第14天和第21天检测到。IL-2、IL-10、IFN-γ和单核细胞趋化蛋白-I在第14天分别增加9.08倍、21天398倍、43.1倍和142倍。第21天，3型胶原、I型胶原和纤连蛋白分别增加34.6倍、1.74倍和44.4倍。有趣的是，骨桥蛋白显示出4540倍的增加，并且在第14天是所有mRNA中最高的。心肌肌球蛋白的一种亚型和NP在EAM中发生了显著变化。RAA系统和ET表达在EAM时间过程中有不同的变化。细胞因子、趋化因子和细胞外基质大大增加，特别是大量增加。骨桥蛋白mRNA在早期EAM中表达。
Genetic responses that characterize experimental autoimmune myocarditis (EAM) have not yet been determined. To investigate gene expression in the myocardium of EAM, absolute copy numbers of 44 mRNA species [calcium-handling proteins, contractile proteins, natriuretic peptides (NPs), cytokines, chemokines, growth factors, renin-angiotensin-aldosterone (RAA) system, endothelins (ETs) and extracellular matrix] in synthesized cDNA from a fixed quantity of total heart RNA were assessed using real-time reverse-transcriptase PCR at days 0, 14, 21 and 28 after immunization. alpha-Cardiac myosin showed a 26.3-fold decrease and beta-cardiac myosin a 3.75-fold increase at day 14. Atrial NP and brain NP increased 47.7- and 6.35-fold at days 21 and 14 respectively. Angiotensin II type I receptor, angiotensin-converting enzyme and ETI increased 22.3-fold at day 21, 6.30-fold at day 21 and 16.8-fold at day 14 respectively. Aldosterone receptor decreased 2.15-fold at day 14, but aldosterone synthetase was detected only at days 14 and 21. Interleukin (IL)-2, IL-10, interferon-gamma and monocyte chemo-attractant protein-I increased 9.08-fold at day 14, 398-fold at day 21, 43.1-fold at day 14 and 142-fold at day 14 respectively. Collagen type 3, collagen type I and fibronectin increased 34.6-, 1.74- and 44.4-fold respectively at day 21. Interestingly, osteopontin showed a 4540-fold increase and it was the highest mRNA of all at day 14. An isoform of cardiac myosin and NP are dramatically changed in EAM. RAA system and ET expressions are changed differently during the EAM time course. Cytokine, chemokine and extracellular matrix greatly increase and, in particular, large numbers. of osteopontin mRNA are expressed in early EAM.