SIMULTANEOUS IDENTIFICATION OF BACTERIAL VIRULENCE GENES BY NEGATIVE SELECTION
SIMULTANEOUS IDENTIFICATION OF BACTERIAL VIRULENCE GENES BY NEGATIVE SELECTION
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DOI：
10.1126/science.7618105
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发表时间：
1995-07-21
期刊：
影响因子：
56.9
通讯作者：
HOLDEN, DW
中科院分区：
文献类型：
--
作者：
HENSEL, M;SHEA, JE;HOLDEN, DW
建立了一种利用带有独特DNA序列标签的转座子进行细菌毒力基因分离的插入突变系统。通过扩增、放射性标记和杂交分析检测来自代表接种物和从感染宿主中回收的细菌的细菌突变体的混合群体的标签。当应用于鼠伤寒沙门氏菌引起的伤寒小鼠模型时，通过使用存在于接种物中但不存在于从受感染小鼠中回收的细菌中的标签来揭示具有减毒毒力的突变体。这种方法导致了新的毒力基因的鉴定，其中一些与S的inv/spa家族相关，但功能不同。鼠伤寒。
An insertional mutagenesis system that uses transposons carrying unique DNA sequence tags was developed for the isolation of bacterial virulence genes. The tags from a mixed population of bacterial mutants representing the inoculum and bacteria recovered from infected hosts were detected by amplification, radiolabeling, and hybridization analysis. When applied to a murine model of typhoid fever caused by Salmonella typhimurium, mutants with attenuated virulence were revealed by use of tags that were present in the inoculum but not in bacteria recovered from infected mice. This approach resulted in the identification of new virulence genes, some of which are related to, but functionally distinct from, the inv/spa family of S. typhimurium.