Amorphous calcium phosphate is a major component of the forming fin bones of zebrafish: Indications for an amorphous precursor phase
Amorphous calcium phosphate is a major component of the forming fin bones of zebrafish: Indications for an amorphous precursor phase
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DOI：
10.1073/pnas.0803354105
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发表时间：
2008-09-02
影响因子：
11.1
通讯作者：
Weiner, Steve
中科院分区：
文献类型：
--
作者：
Mahamid, Julia;Sharir, Amnon;Weiner, Steve
生物矿化中的一个基本问题是第一次形成的矿物相的性质。在脊椎动物的骨形成过程中，这个问题一直备受争议。我们使用图宾根长鳍斑马鱼不断生长的鳍骨射线作为骨骼矿化的模型来解决这一关键问题。利用高分辨率扫描和透射电子显微镜成像、电子衍射和元素分析，我们证明在新形成的鳍骨中存在丰富的无定形磷酸钙相。提取的无定形矿物颗粒随时间结晶，矿物质结晶度在骨成熟过程中增加。基于这些发现，我们认为这种无定形的磷酸钙相可能是后来转变为成熟结晶矿物的前驱相。
A fundamental question in biomineralization is the nature of the first-formed mineral phase. In vertebrate bone formation, this issue has been the subject of a long-standing controversy. We address this key issue using the continuously growing fin bony rays of the Tuebingen long-fin zebrafish as a model for bone mineralization. Employing high-resolution scanning and transmission electron microscopy imaging, electron diffraction, and elemental analysis, we demonstrate the presence of an abundant amorphous calcium phosphate phase in the newly formed fin bones. The extracted amorphous mineral particles crystallize with time, and mineral crystallinity increases during bone maturation. Based on these findings, we propose that this amorphous calcium phosphate phase may be a precursor phase that later transforms into the mature crystalline mineral.