Lipopolysaccharide O-antigen chain length regulation in Pseudomonas aeruginosa serogroup O11 strain PA103
Lipopolysaccharide O-antigen chain length regulation in Pseudomonas aeruginosa serogroup O11 strain PA103
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DOI：
10.1128/jb.01646-07
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发表时间：
2008-04-01
影响因子：
3.2
通讯作者：
Goldberg, Joanna B.
中科院分区：
文献类型：
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作者：
Kintz, Erica;Scarff, Jennifer M.;Goldberg, Joanna B.
Wzz蛋白对于确定附着于脂多糖（LPS）的O-抗原侧链的长度是重要的。几种细菌，包括铜绿假单胞菌菌株PAO 1（血清群05），产生两种这样的蛋白质，负责在表面上的两种不同链长的偏好。我们的研究小组先前已经在铜绿假单胞菌菌株PA 103（血清群O 11）的O-抗原位点内鉴定出一个wzz基因（wzz 1）。在这项研究中，我们已经确定了第二个wzz基因（wzz 2），位于基因组的同一区域，与PAO 1的wzz 2基因有92%的相似性。通过用抗生素抗性盒中断在两个wzz基因中产生突变，并表征这些突变的影响。野生型PA 103偏好两种O-抗原链长度，称为长和非常长。长O-抗原链长度的表达在wzz 1突变体中减少，表明Wzz 1蛋白对于该链长度偏好是重要的。另一方面，wzz 2突变体缺失非常长链长度的O-抗原，表明Wzz 2蛋白负责产生非常长的O-抗原。还研究了wzz突变对毒力的影响。在血清敏感性试验和小鼠肺炎感染模型中，wzz 1突变体与野生型PA 103或wzz 2突变体相比表现出更大的毒力缺陷，表明长链长度在这些感染过程中起着更大的作用。
The Wzz proteins are important for determining the length of the O-antigen side chain attached to lipopolysaccharide (LPS). Several bacteria, including Pseudomonas aeruginosa strain PAO1 (serogroup 05), produce two such proteins responsible for the preference of two different chain lengths on the surface. Our group has previously identified one wzz gene (wzz1) within the O-antigen locus of P. aeruginosa strain PA103 (serogroup O11). In this study we have identified the second wzz gene (wzz2), located in the same region of the genome and with 92% similarity to PAO1's wzz2 gene. Mutations were generated in both wzz genes by interruption with antibiotic resistance cassettes, and the effects of these mutations were characterized. Wildtype PA103 prefers two O-antigen chain lengths, referred to as long and very long. The expression of the long O-antigen chain length was reduced in the wzz] mutant, indicating the Wzz1 protein is important for this chain length preference. The wzz2 mutant, on the other hand, was missing O-antigens of the very long chain length, indicating the Wzz2 protein is responsible for the production of very long O-antigen. The effects of the wzz mutations on virulence were also investigated. In both serum sensitivity assays and a mouse pneumonia model of infection, the wzz1 mutants exhibited greater defects in virulence compared to either wild-type PA103 or the wzz2 mutant, indicating the long chain length plays a greater role during these infectious processes.