Choline and homocysteine interrelations in umbilical cord and maternal plasma at delivery
Choline and homocysteine interrelations in umbilical cord and maternal plasma at delivery
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DOI：
10.1093/ajcn/82.4.836
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发表时间：
2005-10-01
影响因子：
7.1
通讯作者：
Ueland, PM
中科院分区：
文献类型：
--
作者：
Molloy, AM;Mills, JL;Ueland, PM
背景资料：尽管怀孕对母体的叶酸和胆碱储存有相当大的压力，而且胆碱是胎儿的重要营养素，但对怀孕期间胆碱和叶酸以及同型半胱氨酸代谢之间的相互作用知之甚少。胆碱，通过甜菜碱，是一个重要的叶酸非依赖性来源的甲基remethylating homocysteine in liver.Objectives：我们的目的是检查胆碱氧化的中间体在母亲和脐带血浆，并确定这一途径和叶酸依赖性homocysteine remethylation之间的关系。设计：从201名孕妇和分娩时从其健康足月婴儿的脐带静脉中采集血液样本。血浆游离胆碱，甜菜碱，二甲基甘氨酸，叶酸，维生素B-12，总同型半胱氨酸（tHcy），肌酐concentration.Results：脐带血浆中的胆碱浓度约为3倍，在母体血浆（几何x：36.6和12.3 μ mol/L，分别为P < 0.0001）进行了分析。甜菜碱和二甲基甘氨酸浓度也显着高于脐带比母体血浆。母血中胆碱与tHcy（r = 0.34，P < 0.0001）、甜菜碱（r = 0.58，P < 0.0001）和二甲基甘氨酸（r = 0.30，P < 0.0001）呈正相关。在胎儿循环中观察到的关系要弱得多。在多元回归模型中，胆碱是一个积极的预测母体tHcy，而维生素B-12和甜菜碱是负predictor.Conclusions：母亲胆碱和tHcy之间的正相关性在怀孕期间表明，高胎儿需求胆碱刺激从头合成胆碱在母体肝脏，从而增加tHcy浓度。如果这一点得到证实，在怀孕期间提供胆碱补充剂可能是适当的，以防止tHcy浓度升高。
Background: Little is known about the interactions between choline and folate and homocysteine metabolism during pregnancy despite the facts that pregnancy places considerable stress on maternal folate and choline stores and that choline is a critical nutrient for the fetus. Choline, via betaine, is an important folate-independent source of methyl groups for remethylating homocysteine in liver.Objectives: Our aims were to examine the intermediates of choline oxidation in maternal and umbilical cord plasma and to determine the relations between this pathway and folate-dependent homocysteine remethylation.Design: Blood samples were taken from 201 pregnant women and, at delivery, from the umbilical cord veins of their healthy, full-term infants. The blood samples were analyzed for plasma free choline, betaine, dimethylglycine, folate, vitamin B-12, total homocysteme (tHcy), and creatinine concentrations.Results: Choline concentrations in umbilical cord plasma were approximate to 3 times those in maternal plasma (geometric x: 36.6 and 12.3 mu mol/L, respectively; P < 0.0001). Betaine and dimethylglycine concentrations were also significantly higher in umbilical cord than in maternal plasma. Choline was positively associated with tHcy (r = 0.34, P < 0.0001), betaine (r = 0.58, P < 0.0001), and dimethylglycine (r = 0.30, P < 0.0001) in maternal blood. Much weaker relations were seen in the fetal circulation. In a multiple regression model, choline was a positive predictor of maternal tHcy, whereas vitamin B-12 and betaine were negative predictors.Conclusions: The positive association between maternal choline and tHcy during pregnancy suggests that the high fetal demand for choline stimulates de novo synthesis of choline in maternal liver, with a resultant increase in tHcy concentrations. If this is confirmed, it may be appropriate to provide choline supplements during pregnancy to prevent elevated tHcy concentrations.