Norspermine substitutes for thermospermine in the control of stem elongation in Arabidopsis thaliana
Norspermine substitutes for thermospermine in the control of stem elongation in Arabidopsis thaliana
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DOI：
10.1016/j.febslet.2010.05.035
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发表时间：
2010-07-16
期刊：
影响因子：
3.5
通讯作者：
Takahashi, Taku
中科院分区：
文献类型：
--
作者：
Kakehi, Jun-Ichi;Kuwashiro, Yoshitaka;Takahashi, Taku
热精胺是精胺的结构异构体，并且是拟南芥茎伸长所必需的。我们注意到热精胺（C3C3C4）中碳链的C3C3排列，这在精胺（C3C4C3）中不存在，并检查它是否在功能上被去甲精胺（C3C3C3）取代。外源应用norspermine的acl5，在热精胺的合成缺陷的突变体，部分抑制其矮化表型，并下调acl5转录的水平，这是远远高于在野生型的acl5转录。此外，在百日菊培养中，热精胺和去甲精胺对叶肉细胞分化为导管分子有抑制作用，而精胺则无此作用。我们的研究结果表明，去甲精胺可以在功能上取代热精胺。(C)2010年欧洲生物化学学会联合会。Elsevier B.V.出版，保留所有权利。
Thermospermine is a structural isomer of spermine and is required for stem elongation in Arabidopsis thaliana. We noted the C3C3 arrangement of carbon chains in thermospermine (C3C3C4), which is not present in spermine (C3C4C3), and examined if it is functionally replaced with norspermine (C3C3C3) or not. Exogenous application of norspermine to acl5, a mutant defective in the synthesis of thermospermine, partially suppressed its dwarf phenotype, and down-regulated the level of the acl5 transcript which is much higher than that of the ACL5 transcript in the wild type. Furthermore, in the Zinnia culture, differentiation of mesophyll cells into tracheary elements was blocked by thermospermine and norspermine but not by spermine. Our results indicate that norspermine can functionally substitute for thermospermine. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.