Polysaccharide Extracted from Laminaria japonica Delays Intrinsic Skin Aging in Mice.
Polysaccharide Extracted from Laminaria japonica Delays Intrinsic Skin Aging in Mice.
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海带提取的多糖可延缓小鼠内源性皮肤衰老
DOI：
10.1155/2016/5137386
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Tan J
中科院分区：
文献类型：
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作者：
Hu L;Tan J;Yang X;Tan H;Xu X;You M;Qin W;Huang L;Li S;Mo M;Wei H;Li J;Tan J
本研究旨在确定局部应用海带多糖 (LP) 对皮肤老化的影响。我们将含有 LP（10、25 和 50μg/g）或维生素 E（10μg/g）的软膏涂抹在衰老小鼠的背部皮肤上 12 个月，将年轻对照小鼠涂抹 4 周。皮肤样本的电子显微镜分析显示，LP 增加了真皮厚度和皮肤胶原蛋白含量。与未治疗的衰老小鼠相比，LP治疗的皮肤组织中金属蛋白酶组织抑制剂- (TIMP-) 1 的表达上调，而基质金属蛋白酶- (MMP-) 1 的表达下调。此外，老化皮肤中 c-Jun N 末端激酶 (JNK) 和 p38 的磷酸化程度高于年轻皮肤，而 LP 治疗可抑制磷酸化 JNK 表达。与未治疗的衰老小鼠相比，LP的应用还增强了皮肤组织中抗氧化酶的表达，导致丙二醛水平降低，超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶水平增加。这些结果表明，LP 通过防止氧化应激和 JNK 磷酸化来抑制 MMP-1 表达，从而延缓衰老过程中皮肤胶原蛋白的分解。
This study aimed to determine the effect of topically applied Laminaria polysaccharide (LP) on skin aging. We applied ointment containing LP (10, 25, and 50 μg/g) or vitamin E (10 μg/g) to the dorsal skin of aging mice for 12 months and young control mice for 4 weeks. Electron microscopy analysis of skin samples revealed that LP increased dermal thickness and skin collagen content. Tissue inhibitor of metalloprotease- (TIMP-) 1 expression was upregulated while that of matrix metalloproteinase- (MMP-) 1 was downregulated in skin tissue of LP-treated as compared to untreated aging mice. Additionally, phosphorylation of c-Jun N-terminal kinase (JNK) and p38 was higher in aging skin than in young skin, while LP treatment suppressed phospho-JNK expression. LP application also enhanced the expression of antioxidative enzymes in skin tissue, causing a decrease in malondialdehyde levels and increases in superoxide dismutase, catalase, and glutathione peroxidase levels relative to those in untreated aging mice. These results indicate that LP inhibits MMP-1 expression by preventing oxidative stress and JNK phosphorylation, thereby delaying skin collagen breakdown during aging.