Differential expression and regulation of SSTR2 messenger RNA in rat gastric antrum and corpus.
Differential expression and regulation of SSTR2 messenger RNA in rat gastric antrum and corpus.
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DOI：
10.1152/ajpgi.1995.269.4.g542
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发表时间：
1995-10
期刊：
影响因子：
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通讯作者：
A. Sandvik;Rod Dimaline;E. Brenna;Helge L. Waldum
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文献类型：
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作者：
A. Sandvik;Rod Dimaline;E. Brenna;Helge L. Waldum
生长抑素调节胃粘膜的内分泌和外分泌功能，其中五个克隆的生长抑素受体中有三个存在。本研究检测了奥美拉唑在禁食、喂养和深度酸抑制期间生长抑素受体（SSTR） mRNA丰度的变化。测定血清胃泌素、生长抑素和SSTR mRNA在胃窦和体中的丰度。在体RNA的Northern印迹中，SSTR2探针与先前报道的两种mRNA杂交（2.4和2.8 kb）；此外，还检测到先前未报道的1.6 kb弱波段。在腔内，1.6 kb波段占主导地位。空腹使胃窦生长抑素mRNA从100 +/- 8增加到161 +/- 24%，SSTR mRNA从100 +/- 10增加到179 +/- 14% （P < 0.05）。奥美拉唑使胃窦生长抑素mRNA降低至对照组的34 +/- 4% (P < 0.05)，使SSTR mRNA升高至对照组的135 +/- 5% （P < 0.01）。奥美拉唑组体生长抑素mRNA降低至59 +/- 5% (P < 0.05)， SSTR mRNA升高至140 +/- 3% （P < 0.01）。结果表明，胃中存在一种新的SSTR mRNA亚型，并在胃窦中占主导地位。这种SSTR mRNA的丰度在禁食和缺氯时均上调；分别增加或减少内源性胃窦生长抑素的情况。
Somatostatin modulates both endocrine and exocrine functions in the gastric mucosa, where three of the five cloned somatostatin receptors are present. This study examines changes in somatostatin receptor (SSTR) mRNA abundance during fasting, feeding, and profound acid inhibition with omeprazole. Serum gastrin as well as somatostatin and SSTR mRNA abundances were measured in antrum and corpus. In Northern blots of corpus RNA, the SSTR2 probe hybridized with two previously reported species of mRNA (2.4 and 2.8 kb); in addition, a weak previously unreported 1.6-kb band was detected. In antrum, the 1.6-kb band dominated. Fasting increased antral somatostatin mRNA from 100 +/- 8 to 161 +/- 24% and SSTR mRNA from 100 +/- 10 to 179 +/- 14% (P < 0.05). Omeprazole reduced antral somatostatin mRNA to 34 +/- 4% of control (P < 0.05) and elevated SSTR mRNA to 135 +/- 5% of control (P < 0.01). Omeprazole treatment reduced corpus somatostatin mRNA to 59 +/- 5% (P < 0.05), and elevated SSTR mRNA to 140 +/- 3% of control (P < 0.01). The results therefore indicate that a novel SSTR mRNA subtype exists in the stomach and predominates in the antrum. The abundance of this SSTR mRNA is upregulated by both fasting and achlorhydria; conditions that increase or decrease endogenous antral somatostatin, respectively.