Adrenomedullin stimulates interleukin-6 production in Swiss 3T3 cells
Adrenomedullin stimulates interleukin-6 production in Swiss 3T3 cells
复制标题
DOI：
10.1006/bbrc.1998.8261
复制
发表时间：
1998-03-17
影响因子：
3.1
通讯作者：
Matsuo, H
中科院分区：
文献类型：
--
作者：
Isumi, Y;Minamino, N;Matsuo, H
肾上腺髓质素（AM）最近被证明是产生和分泌的成纤维细胞。炎症相关物质可增强成纤维细胞中AAI的产生，Swiss 3 T3成纤维细胞表达AM特异性受体和腺苷酸环化酶。为了评估AM从成纤维细胞分泌的功能，我们测量了AM对Swiss 3 T3细胞中白细胞介素-6（IL-6）（一种参与一般炎症反应的典型细胞因子）产生的影响。AM刺激IL-6的基础分泌5.5倍，而其他肽引起弱得多的刺激作用。AM的这种作用可被AM受体拮抗剂和cAMP依赖性蛋白激酶（PKA）抑制剂所抑制。AM还能显著增强TNF-α、IL-1 β和脂多糖对IL-6产生的刺激作用。AM的这种刺激作用是通过激活基因转录来实现的，在30 min内达到最大值。这些发现证实AM是Swiss 3 T3细胞中通过cAMP-PKA途径作用的IL-6产生的快速且非常有效的调节剂。由此获得的数据表明AM是炎症的肽能调节剂。(C)北京：科学出版社.
Adrenomedullin (AM) has very recently been demonstrated to be produced and secreted from fibroblasts. The production of AAI in the fibroblasts is augmented by inflammation-related substances, and Swiss 3T3 fibroblast cells express AM specific receptors coupled with adenylate cyclase. To assess the functions of AM secreted from fibroblasts, we measured the effect of AM on production in Swiss 3T3 cells of interleukin-6 (IL-6), a typical cytokine involved in the general inflammatory reactions, AM stimulated basal secretion of IL-6 5.5-fold, while other peptides elicited much weaker stimulatory effects. The effect of AM was inhibited with an AM receptor antagonist and a cAMP-dependent protein kinase (PKA) inhibitor, Furthermore, AM remarkably potentiated stimulatory effects of tumor necrosis factor-alpha, IL-1 beta and lipopolysaccharide on IL-6 production, This stimulatory effect of AM was induced through activation of gene transcription, which reached maximum within 30 min, These findings verify that AM is a rapid and extraordinarily potent regulator of IL-6 production in Swiss 3T3 cells acting through the cAMP-PKA pathway, The data thus obtained suggest that AM is a peptidergic regulator of inflammation. (C) 1998 Academic Press.