Ultrasensitive sensor for detection of early stage chronic kidney disease in human
Ultrasensitive sensor for detection of early stage chronic kidney disease in human
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DOI：
10.1016/j.bios.2018.01.031
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发表时间：
2018-05-15
影响因子：
12.6
通讯作者：
Datta, Manali
中科院分区：
文献类型：
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作者：
Desai, Dignya;Kumar, Ashok;Datta, Manali
一个用于快速检测慢性肾脏疾病的简便、无标记、超灵敏的平台已经被制造出来。对慢性肾病患者进行早期干预有可能延缓甚至预防终末期肾病和并发症的发展，从而对预期寿命和生活质量产生显着影响。因此，便携式电化学诊断生物传感器已成为一个有吸引力的选择，因为电化学分析可用于样品的现场检测。在人体中，人体体液中存在的半胱氨酸蛋白酶抑制剂C可被肾小球自由过滤，但被肾小管重吸收和分解代谢。尿液中可检测到的痕量物质被消除，这使得该分子标记比血清肌酐的缺点更具优势。将羧基功能化的多壁碳纳米管丝网印刷电极固定在木瓜蛋白酶（半胱氨酸蛋白酶）上，其中木瓜蛋白酶的氨基通过 EDC（1-乙基-3-（3-二甲基氨基丙基）碳二亚胺）和 NHS（N-羟基琥珀酰亚胺）化学共价结合在电极表面上的羧基。通过场发射扫描电子显微镜对传感器表面的修饰进行了表征。通过循环伏安法和微分脉冲伏安法在 10 分钟内检测到木瓜蛋白酶与慢性肾病特异性生物标志物半胱氨酸蛋白酶抑制剂 C 之间的相互作用。该传感器对半胱氨酸蛋白酶抑制剂 C 具有高度特异性，对尿液中存在的非特异性大分子的反应可以忽略不计。该传感器的灵敏度为1583.49 mu A cm(-2) mu g(-1),Cystatin C的检测下限为0.58 ng L-1,为设计便携式肾脏病检测仪提供了一个有前景的平台。
A facile label free, ultrasensitive platform for a rapid detection of chronic kidney disease has been fabricated. Early intervention in patients with chronic kidney disease has the potential to delay, or even prevent, the development of end stage renal disease and complications, leading to a marked impact on life expectancy and quality of life. Thus, a potable electrochemical diagnostic biosensor has become an attractive option as electrochemical analysis is feasible to use for on-site detection of samples. In human, Cystatin C present in human body fluids is freely filtered by the glomerulus, but reabsorbed and catabolised by the renal tubules. Trace detectable amount is eliminated in urine, giving this molecular marker an edge over serum creatinine's disadvantages. A carboxyl functionalized multiwalled carbon nanotubes screen printed electrode was immobilized with papain (cysteine protease) where amino group of papain covalently bound carboxyl group on electrode surface by EDC (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide) and NHS (N-hydroxysuccinirnide) chemistry. The modifications on sensor surface were characterized by field emission scanning electron microscopy. Interaction between papain and chronic kidney disease specific biomarker, Cystatin C was detected by cyclic voltammetry and differential pulse voltammetry within 10 min. The sensor is highly specific to Cystatin C and showed negligible response to non-specific macromolecules present in urine. The sensitivity of the sensor was 1583.49 mu A cm(-2) mu g(-1) and lower limit of detection of Cystatin C was found 0.58 ng L-1 which presents as a promising platform for designing potable kidney disease detector.