A novel variable delay Go/No-Go task to study attention, motivation and working memory in the head-fixed rodent.
A novel variable delay Go/No-Go task to study attention, motivation and working memory in the head-fixed rodent.
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DOI：
10.12688/f1000research.2-125.v2
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Cooper DC
中科院分区：
文献类型：
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作者：
Dolzani SD;Nakamura S;Cooper DC
为了解析复杂行为和决策过程背后的因果因素，必须开发适当的行为方法，并与分子，药理学和电生理学方法同时使用。提出了一种新的去/不去行为范式的协议，以研究清醒的，头部受限的啮齿动物的大脑注意力和动机/奖励电路。该实验设置允许：（1）通过靶向引导套管对不同脑区进行药理学和病毒操作;（2）光遗传细胞类型特异性激活和沉默，同时进行电生理记录;（3）在持续行为期间重复进行电生理单单位和多单位记录。这项任务包括三个组成部分。受试者首先通过响应于不同的Go或No-Go音调按下杠杆来启动试验，从而做出观察反应。 然后，在可变的延迟期之后，向受试者呈现由白色噪声提示的挑战期，在此期间，受试者必须以杠杆按压响应进行条件或在不进行条件下的提示持续时间内抑制杠杆按压。在激发期（激发）和短暂延迟期间正确响应后，出现与启动音频率相同的最终奖励音，蔗糖奖励输送可用，并取决于杠杆按压。在这里，我们提供了一个新的程序和验证数据集，使研究人员能够在正在进行的操作性行为任务中研究和操纵行为的组成部分，如注意力，动机，冲动和奖励相关的工作记忆，同时限制非任务相关行为的干扰。
In order to parse the causal elements underlying complex behaviors and decision-making processes, appropriate behavioral methods must be developed and used in concurrence with molecular, pharmacological, and electrophysiological approaches. Presented is a protocol for a novel Go/No-Go behavioral paradigm to study the brain attention and motivation/reward circuitry in awake, head-restrained rodents. This experimental setup allows: (1) Pharmacological and viral manipulation of various brain regions via targeted guide cannula; (2) Optogenetic cell-type specific activation and silencing with simultaneous electrophysiological recording and; (3) Repeated electrophysiological single and multiple unit recordings during ongoing behavior. The task consists of three components. The subject first makes an observing response by initiating a trial by lever pressing in response to distinctive Go or No-Go tones. Then, after a variable delay period, the subject is presented with a challenge period cued by white noise during which they must respond with a lever press for the Go condition or withhold from lever pressing for the duration of the cue in the No-Go condition. After correctly responding during the challenge period (Challenge) and a brief delay, a final reward tone of the same frequency as the initiation tone is presented and sucrose reward delivery is available and contingent upon lever pressing. Here, we provide a novel procedure and validating data set that allows researchers to study and manipulate components of behavior such as attention, motivation, impulsivity, and reward-related working memory during an ongoing operant behavioral task while limiting interference from non task-related behaviors.