Rapid identification of B biotype of Bemisia tabaci (Homoptera: Aleyrodidae) based on analysis of internally transcribed spacer 1 sequence
Rapid identification of B biotype of Bemisia tabaci (Homoptera: Aleyrodidae) based on analysis of internally transcribed spacer 1 sequence
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DOI：
10.1111/j.1744-7917.2005.00053.x
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发表时间：
2005-12-01
期刊：
影响因子：
4
通讯作者：
Hu, Dun-Xiao
中科院分区：
文献类型：
--
作者：
Li, Zheng-Xi;Hu, Dun-Xiao
B型是烟粉虱种复合体中较重要的生物型。B的当地人口。从中国大陆、台湾、巴基斯坦和以色列等地采集了烟粉虱在不同寄主植物上的幼虫。从B的每个种群中。烟粉虱的核糖体rDNA基因的内转录间隔区，扩增，克隆和序列测定。序列同源性分析结果与形态学和生物学特性分析结果基本一致。根据内转录间隔区1序列的分析，设计了B生物型特异性引物。PCR诊断结果表明，用正向诊断引物与通用反向引物配对，可通过特异性PCR产物鉴定B生物型。该基于诊断引物的方案可用于初步分析含有B生物型以及其它生物型的混合Bemisia种群。
B biotype is a reasonably important biotype among all known biotypes in the Bemisia tabaci species complex. Local populations of B. tabaci on different host plants were collected from across the Chinese mainland, Taiwan, Pakistan and Israel. From each population of B. tabaci, an internally transcribed spacer region of the ribosomal rDNA gene was amplified, cloned and the sequence determined. Sequence homology analyses were performed and the results were similar to those based on morphology and biological characters. Based on analysis of the internally transcribed spacer 1 sequences, a B biotype-specific primer was designed. The PCR diagnosis results showed that B biotype is identifiable by a specific PCR product by using the forward diagnostic primer paired with a universal reverse primer. This diagnostic primer-based protocol can be used for preliminary analysis of mixed Bemisia populations containing B biotype, as well as other biotypes.