Vitamin D supplementation attenuates oxidative stress in paraspinal skeletal muscles in patients with low back pain
Vitamin D supplementation attenuates oxidative stress in paraspinal skeletal muscles in patients with low back pain
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DOI：
10.1007/s00421-017-3755-1
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发表时间：
2018-01-01
影响因子：
3
通讯作者：
Kaczor, Jan Jacek
中科院分区：
文献类型：
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作者：
Dzik, Katarzyna;Skrobot, Wojciech;Kaczor, Jan Jacek
本研究的目的是评估维生素D缺乏的腰痛患者椎旁肌中氧化应激和维生素D受体的标志物，维生素D水平正常，并在5周后补充维生素D。患者被分为3组：补充维生素D（补充组），安慰剂与正常浓度的维生素D（SUF），和安慰剂组维生素D缺乏（DEF）。在补充维生素D（3200 IU/天，持续5周）之前和之后测量血清维生素D浓度。测定了患者多裂肌中脂质和蛋白质过氧化标记物、抗氧化酶活性和维生素D受体蛋白含量，补充维生素D可提高血清25（OH）D-3水平（p < 0.001）。在脊旁肌中，DEF组的8-异前列腺素和蛋白质羰基含量高于DEF组（p < 0.05）。不同血清维生素D浓度组椎旁肌抗氧化酶活性和维生素D受体均发生变化。补充维生素D组的细胞质超氧化物歧化酶和谷胱甘肽过氧化物酶活性显著高于补充维生素D组（p < 0.05）。不同血清维生素D水平的患者骨骼肌抗氧化酶活性不同。监测氧化应激和VDR蛋白含量可能有助于未来研究维生素D在肌肉中的作用机制。
The aim of the study was to evaluate markers of oxidative stress and vitamin D receptor in paraspinal muscles in low back pain patients with vitamin D deficiency, with normal level of vitamin D, and after 5 weeks of vitamin D supplementation.Patients were divided into three groups: supplemented (SUP) with vitamin D, placebo with normal concentration of vitamin D (SUF), and the placebo group with vitamin D deficiency (DEF). The concentration of serum vitamin D was measured before and after the supplementation with vitamin D (3200 IU/ day for 5 weeks). Markers of lipid and protein peroxidation, the activity of antioxidant enzymes, and protein content of vitamin D receptor was determined in multifidus muscle of patients.Vitamin D supplementation increased serum level of 25(OH)D-3 (p < 0.001). In paraspinal muscle level of 8-isoprostanes and protein carbonyls was higher in DEF group as compared to the SUP group (p < 0.05). Antioxidant enzyme activity and vitamin D receptor in paraspinal muscle altered between the groups with different serum vitamin D concentration. The cytosolic superoxide dismutase and glutathione peroxidase activities were significantly higher in DEF group as compared to the SUP group (p < 0.05).An attenuation of markers of free radical damage of lipids and proteins was observed in participants supplemented with Vitamin D. Antioxidant enzyme activities in skeletal muscle differ among patients with different serum vitamin D concentration. Monitoring oxidative stress and VDR protein content might be useful for future studies on the mechanism(s) of vitamin D action in muscle.