Nanobody Technology: A Versatile Toolkit for Microscopic Imaging, Protein-Protein Interaction Analysis, and Protein Function Exploration.
Nanobody Technology: A Versatile Toolkit for Microscopic Imaging, Protein-Protein Interaction Analysis, and Protein Function Exploration.
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DOI：
10.3389/fimmu.2017.00771
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发表时间：
2017
影响因子：
7.3
通讯作者：
Gettemans J
中科院分区：
文献类型：
--
作者：
Beghein E;Gettemans J
在过去的二十年中，纳米抗体或单域抗体已经在研究，诊断和治疗中找到了自己的方式。衍生自骆驼科动物仅重链抗体的这些抗原结合片段具有显著的特征，这些特征有利于它们在选定的研究领域中优于常规抗体或其片段的用途。在这篇综述中，我们评估了纳米抗体作为基础研究各个方面的研究工具的现状。我们讨论了使用纳米抗体作为荧光显微镜检测试剂，并专注于超分辨率显微镜的最新进展。其次，综述了纳米抗体技术在蛋白质相互作用研究中的应用，重点介绍了其在质谱分析中的可能应用。最后，我们讨论了纳米抗体在研究蛋白质功能方面的潜在价值，并重点介绍了它们最近报道的在靶向蛋白质降解中的应用。在整个审查中，我们强调了最先进的工程策略，可以扩大纳米抗体的多功能性，我们建议未来的应用技术在选定的基础研究领域。
Over the last two decades, nanobodies or single-domain antibodies have found their way in research, diagnostics, and therapy. These antigen-binding fragments, derived from Camelid heavy chain only antibodies, possess remarkable characteristics that favor their use over conventional antibodies or fragments thereof, in selected areas of research. In this review, we assess the current status of nanobodies as research tools in diverse aspects of fundamental research. We discuss the use of nanobodies as detection reagents in fluorescence microscopy and focus on recent advances in super-resolution microscopy. Second, application of nanobody technology in investigating protein–protein interactions is reviewed, with emphasis on possible uses in mass spectrometry. Finally, we discuss the potential value of nanobodies in studying protein function, and we focus on their recently reported application in targeted protein degradation. Throughout the review, we highlight state-of-the-art engineering strategies that could expand nanobody versatility and we suggest future applications of the technology in the selected areas of fundamental research.