HPLC ANALYSIS OF PHENYLTHIOCARBAMYL (PTC) AMINO-ACIDS .1. EVALUATION AND OPTIMIZATION OF THE PROCEDURE
HPLC ANALYSIS OF PHENYLTHIOCARBAMYL (PTC) AMINO-ACIDS .1. EVALUATION AND OPTIMIZATION OF THE PROCEDURE
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DOI：
10.1007/bf02268788
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发表时间：
1986-12-01
期刊：
影响因子：
1.7
通讯作者：
GOVERDE, BC
中科院分区：
文献类型：
--
作者：
JANSSEN, PSL;VANNISPEN, JW;GOVERDE, BC
本文建立了一种高效液相色谱法，可将23种苯硫代氨甲酰基氨基酸（PTC）分离。应用该系统，在HPLC氨基酸分析，即气相水解的蛋白质/肽样品和柱前衍生的前几个步骤，进行了评估和优化。如果在5 ℃下储存，HPLC缓冲溶液中的PTC氨基酸稳定至少16小时。8.degree. C.该方法的灵敏度为低pmol范围。
An HPLC system has been developed in which 23 phenylthiocarbamyl (PTC) amino acids are distinctly separated. Applying this system, the preceding steps in HPLC amino acid analysis i.e. gas-phase hydrolysis of the protein/peptide sample and the pre-column derivatization, were evaluated and optimized. PTC amino acids in HPLC buffer solution are stable for at least 16 hours if stored at 5.degree.-8.degree. C. The sensitivity of the method is the low pmol range.