Aptamer-based colorimetric probe for cocaine
Aptamer-based colorimetric probe for cocaine
复制标题
DOI：
10.1021/ja0259483
复制
发表时间：
2002-08-21
影响因子：
15
通讯作者：
Landry, DW
中科院分区：
文献类型：
--
作者：
Stojanovic, MN;Landry, DW
在2 ~ 600 μM的浓度范围内，抗可卡因适配体与染料二乙基硫代三碳氰配合物作为比色传感器，在760 nm处吸光度衰减。机理研究表明染料的分子间位移是传感器的作用机制。由于染料不溶于缓冲液，可卡因的结合可以用肉眼观察，因为移位的染料沉淀和上清都脱色了。
Complex of an anti-cocaine aptamer and the dye diethylthiotricarbocyanine behaves as a colorimetric sensor with attenuation in absorbance at 760 nm for cocaine in the concentration range of 2−600 μM. Mechanistic studies indicate an intermolecular displacement of the dye as the mechanism of action of the sensor. As the dye is insoluble in buffer, cocaine binding can be followed with the unaided eye as displaced dye precipitates and supernatant decolorizes.