Peptide sequence motif analysis of tandem MS data with the SALSA algorithm
Peptide sequence motif analysis of tandem MS data with the SALSA algorithm
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DOI：
10.1021/ac0155512
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发表时间：
2002-01-01
影响因子：
7.4
通讯作者：
Mason, DE
中科院分区：
文献类型：
--
作者：
Liebler, DC;Hansen, BT;Mason, DE
我们已经开发了一种模式识别算法，称为SALSA（评分算法光谱分析）串联MS（MS-MS）光谱中的特定功能的检测。应用SALSA算法检测肽MS-MS离子系列能够识别显示特定肽序列特征的MS-MS谱。SALSA分析基于肽序列基序的理论离子系列与实际MS-MS产物离子系列之间的对应性对MS-MS谱进行评分，而不管它们在m/z轴上的绝对位置如何。通过LC-MS-MS分析牛血清白蛋白（BSA）的胰蛋白酶解产物，然后进行SALSA分析，检测到几种BSA胰蛋白酶肽的未修饰形式和多种修饰形式的MS-MS光谱。从BSA和人血清白蛋白（HSA）胰蛋白酶消化酶的混合物的MS-MS数据的SALSA分析表明，与BSA肽序列基序的离子系列搜索确定了BSA和密切相关的HSA肽的MS-MS光谱。当SALSA搜索标准针对目标肽进行优化时，实现了变体肽形式的MS-MS谱之间的最佳区分。SALSA应用于LC-MS-MS蛋白质组分析将有助于修饰和序列变异蛋白质的表征。
We have developed a pattern recognition algorithm called SALSA (scoring algorithm for spectral analysis) for the detection of specific features in tandem MS (MS-MS) spectra. Application of the SALSA algorithm to the detection of peptide MS-MS ion series enables identification of MS-MS spectra displaying characteristics of specific peptide sequences. SALSA analysis scores MS-MS spectra based on correspondence between theoretical ion series for peptide sequence motifs and actual MS-MS product ion series, regardless of their absolute positions on the m/z axis. Analyses of tryptic digests of bovine serum albumin (BSA) by LC-MS-MS followed by SALSA analysis detected MS-MS spectra for both unmodified and multiple modified forms of several BSA tryptic peptides. SALSA analysis of MS-MS data from mixtures of BSA and human serum albumin (HSA) tryptic digests indicated that ion series searches with BSA peptide sequence motifs identified MS-MS spectra for both BSA and closely related HSA peptides. Optimal discrimination between MS-MS spectra of variant peptide forms is achieved when the SALSA search criteria are optimized to the target peptide. Application of SALSA to LC-MS-MS proteome analysis will facilitate the characterization of modified and sequence variant proteins.