Microfluidic devices for the generation of centimetre‐long motor nerve organoids derived from iPSCs
Microfluidic devices for the generation of centimetre‐long motor nerve organoids derived from iPSCs
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DOI：
10.1049/mnl.2019.0541
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发表时间：
2020-09
影响因子：
1.3
通讯作者：
S. Kaneda;K. Kurihara;Kenji Suzuki;H. Takanobu;S. Ohka
中科院分区：
文献类型：
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作者：
S. Kaneda;K. Kurihara;Kenji Suzuki;H. Takanobu;S. Ohka
尽管最近报道了利用微通道结构产生由源自人诱导多能干细胞（iPSC）的运动神经元轴突的神经球和束组成的运动神经类器官的微流体神经元培养技术，但轴突束的长度限于小于1cm，其对应于微通道长度。在这项研究中，作者制造了由聚二甲基硅氧烷芯片组成的微流控装置，该芯片具有玻璃基底和20 mm长，150 μ m宽的微通道，以获得厘米长的轴突束。他们通过使用三种不同的微通道高度（120，150和220 μm），含有80，000个来自人类iPSCs细胞系（409B2）的运动神经元细胞的神经球和N2B27培养基来研究微通道高度对纤维束长度的影响。使用150 μ m高的微通道，成功地实现了分别为2和1.25 cm的最大和平均纤维束长度。随着培养天数的增加（每天0.44至0.60 mm），束的长度增加，在培养的第23天左右生长至超过1 cm。用120 μm和150 μ m高的微通道获得的纤维束长度的差异表明，高度显著影响神经类器官轴突纤维束的长度。
Although a microfluidic neuronal culture technique that utilised a microchannel structure to generate a motor nerve organoid consisted of neurospheres and fascicles of motor neuronal axons derived from human induced pluripotent stem cells (iPSCs) was reported recently, the lengths of the axonal fascicles were limited to less than 1 cm, which corresponded to the microchannel length. In this study, the authors fabricated microfluidic devices composed of a polydimethylsiloxane chip with a glass substrate and 20-mm-long and 150-μm-wide microchannels to obtain centimetre-long axonal fascicles. They investigated the effects of microchannel height on fascicle length by using three different microchannel heights (120, 150, and 220 μm), neurospheres containing 80,000 motor neuronal cells derived from a human iPSCs cell line (409B2), and N2B27 medium. Maximum and average fascicle lengths of 2 and 1.25 cm, respectively, were successfully achieved with 150-μm-high microchannels. The fascicles grew in length, with an increase in the number of days spent in the culture (ranging from 0.44 to 0.60 mm per day), growing to over 1 cm around the 23rd day of culture. The difference in fascicle length obtained with 120-μm and 150-μm-high microchannels suggests that height significantly affects the length of the axonal fascicle of the nerve organoid.