Evaluation of the pathogenicity of Listeria spp. in Caenorhabditis elegans
Evaluation of the pathogenicity of Listeria spp. in Caenorhabditis elegans
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DOI：
10.1089/fpd.2006.64
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发表时间：
2007-03-01
影响因子：
2.8
通讯作者：
Schwab, Ute
中科院分区：
文献类型：
--
作者：
Forrester, Stacyann;Milillo, Sara Rose;Schwab, Ute
小杆线虫已被证明是研究宿主-病原体相互作用以及线虫作为食源性病原体传播载体的能力的有用模型。在这项研究中，我们评估是否C。秀丽线虫可以作为李斯特菌属的宿主。培养基对C. elegans killing、C. elegans暴露于L.单核细胞增多症和L.与非致病性E. coliOP 50，而L. seeligeri的存活率与E. coli OP 50。在偏好性测定中，C. elegans偏爱E.大肠杆菌对L.单核细胞增多症和L. innocua，但对L.单核细胞增多症和L.无害。庆大霉素试验表明L.单核细胞增多症在C. elegans肠道我们的研究结果表明，L.单核细胞增多症和L.测试的无害菌株对C. elegans和L.单核细胞增多症没有建立肠道感染与其他最近发表的结果，发现肠道感染和杀死C。elegans，L.单核细胞增多症因此，需要进一步的研究来阐明L。单核细胞增多症和C.包括环境条件和菌株差异对杀灭和肠道感染的影响。
Caenorhabditis has proven to be a useful model for studying host-pathogen interactions as well as the ability of nematodes to serve as vectors for the dispersal of foodborne pathogens. In this study, we evaluated whether C. elegans can serve as a host for Listeria spp. While there was an effect of growth media on C. elegans killing, C. elegans exposed to L. monocytogenes and L. innocua pregrown in Luria-Bertani medium showed reduced survival when compared to nonpathogenic E. coli OP50, while L. seeligeri showed survival similar to E. coli OP50. In a preference assay, C. elegans preferred E. coli over L. monocytogenes and L. innocua, but showed no preference between L. monocytogenes and L. innocua. A gentamicin assay indicated that L. monocytogenes did not persist within the C. elegans intestinal tract. Our findings that L. monocytogenes and L. innocua strains tested have equally deleterious effects on C. elegans and that L. monocytogenes did not establish intestinal infection conflict with other recently published results, which found intestinal infection and killing of C. elegans by L. monocytogenes. Further studies are thus needed to clarify the interactions between L. monocytogenes and C. elegans, including effects of environmental conditions and strain differences on killing and intestinal infection.