Immunohistochemical localization of N-methyl-D-aspartate receptor subunits in the adult murine hippocampal formation: evidence for a unique role of the NR2D subunit
Immunohistochemical localization of N-methyl-D-aspartate receptor subunits in the adult murine hippocampal formation: evidence for a unique role of the NR2D subunit
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DOI：
10.1016/s0169-328x(02)00183-3
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发表时间：
2002-06-15
期刊：
影响因子：
--
通讯作者：
Chazot, PL
中科院分区：
文献类型：
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作者：
Thompson, CL;Drewery, DL;Chazot, PL
从成年小鼠前脑中免疫纯化NMDA受体，免疫印迹法筛选出NR1与NR2A、NR2B和NR2D相关，但不与NR2C相关，在成年小鼠海马膜中也未检测到NR2C。定位了NRI、2A、2B和2D在成年小鼠海马区的解剖分布。NR1样免疫反应定位于锥体神经元胞体、齿状回颗粒细胞和门细胞。CA亚区的顶树突和肺门细胞也呈免疫阳性。NR2A和NR2B样免疫反应基本上与NRI共同定位，暗示这一大脑结构中的所有三个亚单位共同组装。NR2D样免疫反应明显，完全排除锥体、颗粒和Hilax细胞体。与突触前受体的标记一致，在CA1和CA3的透明层有较强的点状染色，在齿状回分子层的内1/3也有较弱的染色。(C)2002 Elsevier Science B.V.保留所有权利。
NMDA receptors were immunopurified from adult mouse forebrain and screened by immunoblotting, NR1 was co-associated with NR2A, NR2B and NR2D but not NR2C, nor was NR2C detected in adult mouse hippocampal membranes. The anatomical distribution of NRI, 2A, 2B and 2D was mapped in the adult murine hippocampal formation. NR1-like immunoreactivity was localised to cell bodies of pyramidal neurons, granule cells and hilar cells of the dentate gyrus. Apical dendrites of the CA subfields and hilar cells were also immunopositive. NR2A- and NR2B-like immunoreactivity essentially co-localised with that of NRI implying co-assembly of all three subunits in this brain structure. NR2D-like immunoreactivity was distinct, being totally excluded from pyramidal, granule and hilax cell bodies. Strong, punctate staining was restricted to the oriens layer of CA1 and the stratum lucidum of CA3 consistent with labelling of presynaptic receptors, Less intense staining was also observed in the internal third of the molecular layer of the dentate gyrus. (C) 2002 Elsevier Science B.V. All rights reserved.