Transcriptome analysis of the venom glands of the Chinese wolf spider Lycosa singoriensis
Transcriptome analysis of the venom glands of the Chinese wolf spider Lycosa singoriensis
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中国狼蛛Lycosa singoriensis毒腺的转录组分析
DOI：
10.1016/j.zool.2009.04.001
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发表时间：
2010-01-01
期刊：
影响因子：
2
通讯作者：
Liang, Songping
中科院分区：
文献类型：
--
作者：
Zhang, Yongqun;Chen, Jinjun;Liang, Songping
狼蛛Lycosasingoriensis是一种广泛分布于我国西北地区的狩猎蜘蛛。蜘蛛的毒腺是一种通气特化的分泌组织，能分泌丰富而复杂的毒素成分。目的：研究L.从定向cDNA文库中获得了833个表达序列标签（expressed sequence tags，EST）。毒素样序列占69.1%，17.3%与细胞转录本相似，13.6%与已知序列无明显相似性。在这里，我们确定了223个新的毒素样序列，可以分为六个不同的。超家族;这意味着发现了各种离子通道的配体的新的潜在来源。借助于基因本体论术语和与真核生物同源性组的同源性，细胞转录物的注释揭示了一些对毒腺的毒素分泌重要的细胞过程，包括蛋白质合成、蛋白质折叠、调谐的翻译后加工和运输等（C）2009 Elsevier GmbH。All rights reserved.
The wolf spider Lycosa singoriensis is a hunting spider with a widespread distribution in northwest China. The venom gland of spiders, which is a vent specialized secretory tissue, can secrete abundant and complex toxin components. To extensively examine the transcripts expressed in the venom glands of L. singoriensis, we generated 833 expressed sequence tags (ESTs) from a directional cDNA library. Toxin-like sequences account for 69.1% of these ESTs, 17.3% are similar to cellular transcripts and 13.6% have no significant similarity to any known sequences. Here, we identified 223 novel toxin-like sequences, which can be classified into six different. superfamilies; that means a novel potential source of ligands for varied ion channels was discovered. With the aid of Gene Ontology terms and homology to eukaryotic orthologous groups, the annotation of cellular transcripts revealed some cellular processes important for the toxin secretion of venom glands including protein synthesis, protein folding, tuned post-translational processing and trafficking, etc. (C) 2009 Elsevier GmbH. All rights reserved.