Structure-dependent retention of steroid hormones by common laboratory materials.
Structure-dependent retention of steroid hormones by common laboratory materials.
复制标题
常见实验室材料对类固醇激素的结构依赖性保留。
DOI：
10.1016/j.jsbmb.2019.105572
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Sharifi,Nima
中科院分区：
文献类型：
--
作者：
McManus,JeffreyM;Sharifi,Nima
类固醇分子吸附在各种材料，特别是塑料上的倾向，几十年前就已为人所知，但在科学界没有得到广泛关注，而且缺乏现代的、系统的研究。对于研究类固醇激素的研究人员来说，这种吸附是一个重要的考虑因素，因为它可能会扭曲各种实验的结果。在这里，我们展示了类固醇吸附在实验中使用的各种容器上，包括微离心管、玻璃小瓶和细胞培养板，其吸附方式取决于类固醇的分子结构和容器类型。类固醇的亲脂性是吸附程度的一个强有力的预测指标，近50%的亲脂性类固醇，孕烯醇酮，在孕烯醇酮水溶液孵卵一小时后，在去除水溶液溶剂后，保留在高吸附性的微离心管中。我们还展示了其他因素如孵育时间、离心和温度对吸附的影响，并表明吸附可以通过类固醇溶液中血清蛋白的存在和/或通过使用低吸附管来防止。
The tendency of steroid molecules to adsorb to various materials, particularly plastics, has been known of for decades but has not received widespread attention in the scientific community, and a modern, systematic study is lacking. This adsorption is an important consideration for researchers working with steroid hormones as it could skew the results of various experiments. Here we show that steroids adsorb to various vessels used in experiments, including microcentrifuge tubes, glass vials, and cell culture plates, in a manner that depends on the steroid’s molecular structure and on the type of vessel. The lipophilicity of steroids is a strong predictor of the degree of adsorption, with nearly 50 % of the most lipophilic steroid tested, pregnenolone, retained in a high-adsorbing microcentrifuge tube after one hour incubation of an aqueous pregnenolone solution followed by removal of the aqueous solvent. We also show the effects of other factors such as incubation time, centrifugation, and temperature on adsorption, and show that adsorption can be mostly prevented by the presence of serum proteins in steroid solutions and/or by the use of low-adsorbing tubes.