The in vivo quantitation and kinetics of monocyte migration into acute inflammatory tissue.
The in vivo quantitation and kinetics of monocyte migration into acute inflammatory tissue.
复制标题
单核细胞迁移到急性炎症组织的体内定量和动力学。
作者：
T. Issekutz;A. Issekutz;H. Movat
从家兔血液中分离单核白细胞，用51 Cr标记并静脉内返回动物。51 Cr标记的单核细胞从血液中消失，半衰期为39.0 +/- 2.51小时。将大肠杆菌皮内注射到家兔背部皮肤中，产生了许多急性炎性损伤。1小时后处死动物，并测定每个病灶中的放射性。单核细胞积累是大量的时间病变是1小时。最大蓄积速率出现在3- 4小时，单核细胞继续以最大速率的25%进入病变至少24小时。单核细胞最初与中性粒细胞同时迁移到细菌炎症部位，12小时后在组织学上成为主要的细胞类型，因为它们在中性粒细胞停止后很长时间内继续迁移到这些病变中。单核细胞迁移的动力学与炎症的其他方面有关。
Mononuclear leukocytes isolated from the blood of rabbits, were labeled with 51Cr and returned to the animal intravenously. 51Cr-labeled monocytes disappeared from the blood with a half-life of 39.0 +/- 2.51 hours. Numerous acute inflammatory lesions were produced by the intradermal injection of Escherichia coli into the skin of the back of a rabbit. The animal was sacrificed after 1 hour, and the radioactivity in each lesion was determined. Monocyte accumulation was substantial by the time a lesion was 1 hour old. The maximum rate of accumulation occurred at 3--4 hours, and monocytes continued to enter the lesions at 25% of the maximal rate for at least 24 hours. Monocytes initially migrate into bacterial inflammatory sites simultaneously with neutrophils and histologically become the predominant cell type after 12 hours because they continue to migrate into these lesions long after neutrophils have stopped. The kinetics of monocyte migration is related to other aspects of inflammation.